Moleón Borodowsky I
Instituto de Medicina Tropical Pedro Kouri.
Rev Cubana Med Trop. 1992;44(2):158-62.
A study was conducted on the parasitemia detection level and the specificity of the pRepHind DNA probe for diagnosing Plasmodium falciparum by the stain hybridization method. The parasitemia detection level was studied by using dilutions of a P. falciparum in vitro culture, adjusted by direct microscopic examination to 1; 0.1; 0.01; 0.001; 0.0001 and 0.00001% of parasited red cells. Specificity was increased by using DNA extractions from P. Yoelii, P. berghei and human leucocytes. The results showed that the method was able to detect 0.0001% of parasitemia starting from DNA extractions of 100 L infected red cells. The pRepHind probe only detected specifically DNA from P. falciparum. It is concluded that the method is suitable for being used in the diagnosis of infection due to P. falciparum.
通过染色杂交法对疟原虫血症检测水平和pRepHind DNA探针诊断恶性疟原虫的特异性进行了研究。通过使用恶性疟原虫体外培养物的稀释液来研究疟原虫血症检测水平，通过直接显微镜检查将其调整为1%、0.1%、0.01%、0.001%、0.0001%和0.00001%的寄生红细胞。通过使用约氏疟原虫、伯氏疟原虫和人类白细胞的DNA提取物来提高特异性。结果表明，该方法从100微升感染红细胞的DNA提取物中能够检测到0.0001%的疟原虫血症。pRepHind探针仅特异性地检测到恶性疟原虫的DNA。得出的结论是，该方法适用于诊断恶性疟原虫感染。
