Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Eur Rev Med Pharmacol Sci. 2016 Mar;20(5):853-7.
To investigate the JNK/AP-1 signaling pathway of airway mucus hypersecretion of severe pneumonia under respiratory virus (RSV) infection.
Total of 56 severe pneumonia children under RSV infection were selected. Reverse transcription polymerase chain reaction (RT-PCR) was performed to measure the expression quantity of MUC5B mRNA and MUC5AC mRNA, and ELISA was used to measure the expression quantity of MUC5AC and MUC5B proteins. Following that, the children were divided into airway mucus hypersecretion group (n = 37) and non-hypersecretion group (n = 19). Western blotting was performed to detect the expression levels of JNK1/2, p-JNK1/2 and AP-1 proteins.
Expression of MUC5AC and MUC5B proteins, and MUC5AC mRNA and MUC5B mRNA in the airway mucus hypersecretion group were significantly higher than those in the non-hypersecretion group (p < 0.05). The expression levels of JNK1/2, p-JNK1/2 and AP-1 proteins in airway mucus hypersecretion group were higher than those in the non-hypersecretion group (p < 0.05).
MUC5AC and MUC5B can be used as marker molecules of airway mucus hypersecretion. Airway mucus hypersecretion of severe pneumonia induced by RSV might be related to the activation of JNK/AP-1 signaling pathway.
研究呼吸道合胞病毒（RSV）感染下重症肺炎气道黏液高分泌的 JNK/AP-1 信号通路。
选择 56 例 RSV 感染下的重症肺炎患儿，采用逆转录聚合酶链反应（RT-PCR）测定 MUC5BmRNA 和 MUC5ACmRNA 的表达量，酶联免疫吸附法（ELISA）测定 MUC5AC 和 MUC5B 蛋白的表达量。根据气道黏液高分泌情况将患儿分为气道黏液高分泌组（n=37）和非高分泌组（n=19）。采用 Western blot 检测 JNK1/2、p-JNK1/2 和 AP-1 蛋白的表达水平。
气道黏液高分泌组的 MUC5AC 和 MUC5B 蛋白及 MUC5ACmRNA 和 MUC5BmRNA 表达水平明显高于非高分泌组（p<0.05）。气道黏液高分泌组 JNK1/2、p-JNK1/2 和 AP-1 蛋白的表达水平高于非高分泌组（p<0.05）。
MUC5AC 和 MUC5B 可作为气道黏液高分泌的标志物。RSV 诱导的重症肺炎气道黏液高分泌可能与 JNK/AP-1 信号通路的激活有关。
