Motallebi Riekandeh S, Mazandarani M, Ebrahimzadeh M A, Zargari M
Department of Biology, Science and Research Branch, Islamic Azad University, Fars, Iran.
Eur Rev Med Pharmacol Sci. 2016 Mar;20(5):946-9.
Medicinal plants are good sources for discovery of safe and new antioxidants. The aim of present study was to evaluate the efficiencies of three methods for evaluated for antioxidants from Eryngium caucasicum.
Ultrasonically assisted extraction, Soxhlet extraction and percolation method were evaluated for the extraction of antioxidants from E. caucasicum inflorescence. Antioxidant activities of extracts were evaluated with four different in vitro tests.
Soxhlet extract had higher total phenolic and flavonoid contents than other extracts. Soxhlet extract showed the highest activity in DPPH radical scavenging (IC50 = 83.1 ± 2.1 µg ml-1). Soxhlet extract showed the best activity in iron chelatory (IC50 = 272 ± 6.3 µg ml-1) followed by ultrasonic extract. Percolation extract showed higher NO radical scavenging (IC50 = 390 ± 11.4 µg ml-1) than other extracts. Extracts showed good reducing power (p > 0.05).
The results obtained indicated that all three extraction methods especially Soxhlet method could effectively extract antioxidants from this plant.
药用植物是发现安全新型抗氧化剂的良好来源。本研究的目的是评估从高加索刺芹中提取抗氧化剂的三种方法的效率。
对超声辅助提取法、索氏提取法和渗漉法从高加索刺芹花序中提取抗氧化剂的效果进行了评估。用四种不同的体外试验评估提取物的抗氧化活性。
索氏提取物的总酚和黄酮含量高于其他提取物。索氏提取物在DPPH自由基清除试验中表现出最高活性（IC50 = 83.1 ± 2.1 µg ml-1）。索氏提取物在铁螯合试验中表现出最佳活性（IC50 = 272 ± 6.3 µg ml-1），其次是超声提取物。渗漉提取物在NO自由基清除试验中表现出比其他提取物更高的活性（IC50 = 390 ± 11.4 µg ml-1）。提取物表现出良好的还原能力（p > 0.05）。
所得结果表明，所有三种提取方法，尤其是索氏提取法，都能有效地从该植物中提取抗氧化剂。
