高加索蓝刺头总黄酮部位的生物活性研究

Biological activities of flavonoid-rich fraction of Eryngium caucasicum Trautv.

机构信息

Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

出版信息

Eur Rev Med Pharmacol Sci. 2012 Jul;16 Suppl 3:81-7.

Abstract

OBJECTIVES

Eryngium (E.) caucasicum was found as a new cultivated vegetable plant in northern Iran and used in several local foods. Little information is available on biological properties of E. caucasicum. In this work antioxidant activity of flavonoid-rich fraction of this plant was investigated by eight in vitro assay systems.

MATERIALS AND METHODS

1,1-diphenyl-2-picryl hydrazyl radical (DPPH), nitric oxide and hydrogen peroxide scavenging activities, Fe2+ chelating ability, reducing power and hemoglobin-induced linoleic acid peroxidation were used to evaluate antioxidant activities. Antihemolytic activities were evaluated against CuOOH and H2O2 induced hemolysis in rat erythrocyte.

RESULTS

IC50 for DPPH scavenging activity was 391.2 +/- 14.9, 706.6 +/- 22.3 and 779.7 +/- 16.7 microg ml(-1) for aqueous (AQ), ethyl acetate (EA) and n-hexane (HE) fractions, respectively. There was no difference between reducing power of AQ fraction activity and vitamin C (p > 0.05). IC50 for NO radical-scavenging activity was in order of AQ (133.5 +/- 6.2 microg ml(-1)) > EA (350.1 +/- 14.8 microg ml(-1)) > and HE (639.9 +/- 21.7 microg ml(-1)) fractions, respectively. Extracts showed weak Fe2+ chelating ability. HE fraction showed better activity (173.5 +/- 9.6 microg ml(-1)). Extracts exhibited weak hydrogen peroxide scavenging activity but exhibited very good antioxidant activity against the hemoglobin-induced linoleic acid peroxidation. There was no significant difference between AQ fraction and vitamin C (p > 0.01). Fractions delayed the onset of the CuOOH induced hemolysis. AQ fraction showed very high total phenol and flavonoid contents which was higher than other fractions. High phenol and flavonoid content of AQ fraction may lead to its better antioxidant activity.

CONCLUSIONS

E. caucasicum fractions exhibited different levels of antioxidant and antihemolytic activities in all tested models. These results can be useful as a starting point of view for further applications of E. caucasicum aerial parts or its constituents in pharmaceutical preparations.

摘要

目的

在伊朗北部发现一种新的蔬菜作物——岩蔷薇（E. caucasicum），并将其用于多种当地食品中。关于 E. caucasicum 的生物特性的信息很少。在这项工作中，通过八种体外测定系统研究了该植物富含类黄酮的部分的抗氧化活性。

材料和方法

使用 1,1-二苯基-2-苦基肼基（DPPH）自由基、一氧化氮和过氧化氢清除活性、Fe2+螯合能力、还原力和血红蛋白诱导的亚油酸过氧化来评估抗氧化活性。抗溶血活性是针对 CuOOH 和 H2O2 诱导的大鼠红细胞溶血来评估的。

结果

DPPH 清除活性的 IC50 分别为 391.2±14.9、706.6±22.3 和 779.7±16.7μg ml-1，对于水（AQ）、乙酸乙酯（EA）和正己烷（HE）部分。AQ 部分的还原力活性与维生素 C 之间没有差异（p>0.05）。NO 自由基清除活性的 IC50 顺序为 AQ（133.5±6.2μg ml-1）>EA（350.1±14.8μg ml-1）>HE（639.9±21.7μg ml-1）。提取物显示出较弱的 Fe2+螯合能力。HE 部分表现出更好的活性（173.5±9.6μg ml-1）。提取物对过氧化氢的清除活性较弱，但对血红蛋白诱导的亚油酸过氧化具有很好的抗氧化活性。AQ 部分与维生素 C 之间没有显著差异（p>0.01）。各部分均延迟了 CuOOH 诱导的溶血的发生。AQ 部分表现出非常高的总酚和类黄酮含量，高于其他部分。AQ 部分高酚和高类黄酮含量可能导致其更好的抗氧化活性。