Meliani Hayate, Makhloufi Ahmed, Cherif Ameur, Mahjoubi Mouna, Makhloufi Khadidja
Laboratory of Valorization of Vegetal Resource and Food Security in Semi Arid Areas, South West of Algeria, BP 417, University of Bechar, Algeria.
Laboratory of Biotechnology and Valorization of Bio - Geo-Resources LR11ES31, Higher Institute of Biotechnology - Sidi ThabetBiotechpole Sidi Thabet, University of Manouba, Ariana, Tunisia.
Saudi J Biol Sci. 2022 Jun;29(6):103288. doi: 10.1016/j.sjbs.2022.103288. Epub 2022 Apr 21.
Fungi colonizing fruits in the field and post-harvest constitute a major threat to the global food sector. This study focuses on the biocontrol of (aflatoxin-producing mold considered carcinogenic by IARC) and f. sp. albedinis (FOA) (phytopathogenic agent, causal of El Bayoud in the Algerian and Moroccan Sahara). These molds have a significant economic impact and pose a serious human health problem. The aim of this work is to study the antifungal activity of two rare actinomycetes strains; sp. COL22 and sp. COL08 strains against toxinogenic and . f. sp. albedinis. The strains are isolated from rhizosphere on different media: ISP2, GLM, TSA, Starch-casein-agar and WYE and with different treatments of the samples (physical, chemical treatment and enrichment). The antifungal tests against the pathogenic microorganisms were performed on ISP2, GLM and TSA medium by means of the agar cylinders method. The kinetics of antibiotic production were performed on ISP medium over 16 days. The characterization of the antimicrobial compounds by LC-ESI/MS-MS showed that the bacterial extracts contain Antibiotic SF 2738C, Tetrodecamycin and Aplysillamide B. The phenotypic and molecular studies showed that sp. COL22 is closely related to the strain type and that sp. COL08 is closely related to the strain type. The two strains are rare and showed an interesting activity against toxinogenic and f. sp. albedinis.
在田间和收获后侵染果实的真菌对全球食品行业构成了重大威胁。本研究聚焦于对黄曲霉（被国际癌症研究机构认定为致癌的产黄曲霉毒素霉菌）和阿氏帚霉f. sp. albedinis（植物病原体，是阿尔及利亚和摩洛哥撒哈拉地区椰枣黑腐病的病因）的生物防治。这些霉菌具有重大经济影响，并造成严重的人类健康问题。这项工作的目的是研究两株稀有放线菌菌株的抗真菌活性；链霉菌属COL22菌株和链霉菌属COL08菌株对产毒素的黄曲霉和阿氏帚霉f. sp. albedinis的抗真菌活性。这些菌株是从不同培养基（ISP2、GLM、TSA、淀粉酪蛋白琼脂和WYE）上的植物根际分离得到的，且样品经过了不同处理（物理、化学处理和富集）。通过琼脂柱法在ISP2、GLM和TSA培养基上对致病微生物进行了抗真菌试验。在ISP培养基上进行了为期16天的抗生素产生动力学研究。通过LC - ESI/MS - MS对抗菌化合物进行表征表明，细菌提取物含有抗生素SF 2738C、十四元霉素和海兔酰胺B。表型和分子研究表明，链霉菌属COL22与链霉菌菌株类型密切相关，链霉菌属COL08与链霉菌菌株类型密切相关。这两株菌株较为稀有，且对产毒素的黄曲霉和阿氏帚霉f. sp. albedinis表现出了有趣的活性。
