基于链置换介导的循环利用对高浓度细胞裂解物中端粒酶活性进行荧光检测。
Fluorescence detection of telomerase activity in high concentration of cell lysates based on strand-displacement mediated recycling.
作者信息
Li Xiaoqian, Wang Wei, Chen Yaoyao, Ding Caifeng
机构信息
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China.
出版信息
Analyst. 2016 Apr 21;141(8):2388-91. doi: 10.1039/c5an02560e. Epub 2016 Mar 24.
PMID:27010225
Abstract
Based on a template of the telomere elongation reaction of a telomerase substrate primer in the presence of telomerase, the strand-displacement process was used to prepare a fluorescence signal nanoprobe. An amplified fluorescence detection of telomerase activity in cancer cells is described in this manuscript.
摘要
基于端粒酶存在下端粒酶底物引物的端粒延伸反应模板，采用链置换过程制备了荧光信号纳米探针。本文描述了癌细胞中端粒酶活性的荧光放大检测。
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