Henriques Ana M, Fagulha Teresa, Duarte Margarida, Ramos Fernanda, Barros Sílvia C, Luís Tiago, Bernardino Rui, Fernandes Teresa L, Lapão Narciso, da Silva José Ferreira, Fevereiro Miguel
J Zoo Wildl Med. 2016 Mar;47(1):161-74. doi: 10.1638/2011-0101.1.
Avian poxviruses (APV) are very large viruses spread worldwide in a variety of hosts. They are responsible for a disease usually referred to as pox, mainly characterized by nodular lesions on feather-free regions of the body. On May 2010, a young American flamingo (Phoenicopterus ruber) of the Lisbon Zoo (Portugal) developed a nodular lesion suggestive of poxvirus infection on its right foot. Avipoxvirus was isolated from the lesion and a fragment of the P4b-encoding gene was amplified by polymerase chain reaction. The nucleotide sequence of the amplicon was determined and analyzed. A close relationship (100% identity) was observed between the flamingo poxvirus and isolates from great bustard (Hungary 2005), house sparrow (Morocco 2009), MacQueen's bustard (Morocco 2011), and Houbara bustard (Morocco 2010 and 2011), suggesting interspecies transmission as a possible source of infection. To strengthen the investigation, the 5' and 3' ends of genes cnpv186 and cnpv 187, respectively, were also analyzed. The cnpv186-187 fragment exhibited 100% identity with MacQueen's bustard and Houbara bustard isolates, both from Morocco 2011. Phylogenetic analyses based in both fragments grouped the flamingo isolate consistently within clade B2 of canarypox. However, the phylogenetic relationships among the different representatives of avian poxviruses were more comprehensive in the tree based on the concatenated coding sequences of the cnpv186-187 fragment, rather than on the P4b-coding gene. The clearer displacement and distribution of the isolates regarding their host species in this last tree suggests the potential usefulness of this genomic region to refine avian poxvirus classification.
禽痘病毒（APV）是一类非常大的病毒，在全球范围内的多种宿主中传播。它们引发一种通常被称为痘的疾病，主要特征是在身体无毛部位出现结节性病变。2010年5月，葡萄牙里斯本动物园的一只美洲火烈鸟幼鸟（Phoenicopterus ruber）右脚上出现了一个提示痘病毒感染的结节性病变。从该病变中分离出禽痘病毒，并通过聚合酶链反应扩增了编码P4b的基因片段。测定并分析了扩增子的核苷酸序列。观察到火烈鸟痘病毒与大鸨（匈牙利，2005年）、家麻雀（摩洛哥，2009年）、毛腿沙鸡（摩洛哥，2011年）和蓑羽鹤（摩洛哥，2010年和2011年）的分离株之间存在密切关系（100%同一性），这表明种间传播可能是感染源。为加强调查，还分别分析了基因cnpv186和cnpv 187的5'端和3'端。cnpv186 - 187片段与均来自2011年摩洛哥的毛腿沙鸡和蓑羽鹤分离株具有100%同一性。基于这两个片段的系统发育分析将火烈鸟分离株一致地归为金丝雀痘病毒的B2分支内。然而，基于cnpv186 - 187片段的串联编码序列构建的树中，禽痘病毒不同代表株之间的系统发育关系比基于P4b编码基因构建的树更全面。在最后一棵树上，分离株相对于其宿主物种更清晰的分布和排列表明该基因组区域在完善禽痘病毒分类方面具有潜在用途。
