Goñi Oscar, Fort Antoine, Quille Patrick, McKeown Peter C, Spillane Charles, O'Connell Shane
Plant Biostimulant Group, Shannon Applied Biotechnology Centre, Institute of Technology , Tralee, Co. Kerry, Ireland.
Genetics and Biotechnology Laboratory, Plant and AgriBiosciences Research Centre (PABC), National University of Ireland Galway , University Road, Galway H91 REW4, Ireland.
J Agric Food Chem. 2016 Apr 13;64(14):2980-9. doi: 10.1021/acs.jafc.6b00621. Epub 2016 Apr 1.
Biostimulants for crop management are gaining increased attention with continued demand for increased crop yields. Seaweed extracts represent one category of biostimulant, with Ascophyllum nodosum extracts (ANE) widely used for yield and quality enhancement. This study investigated how the composition of two ANE biostimulants (ANE A and ANE B) affects plant mRNA transcriptomes, using the model plant Arabidopsis thaliana. Using Affymetrix Ath1 microarrays, significant heterogeneity was detected between the ANE biostimulants in terms of their impacts on the mRNA transcriptome of A. thaliana plants, which accumulated significantly more biomass than untreated controls. Genes dysregulated by the ANE biostimulants are associated with a wide array of predicted biological processes, molecular functions, and subcellular distributions. ANE A dysregulated 4.47% of the transcriptome, whereas ANE B dysregulated 0.87%. The compositions of both ANEs were significantly different, with a 4-fold difference in polyphenol levels, the largest observed. The standardization of the composition of ANE biostimulants represents a challenge for providing consistent effects on plant gene expression and biostimulation.
随着对作物产量提高的持续需求，用于作物管理的生物刺激剂越来越受到关注。海藻提取物是生物刺激剂的一类，其中泡叶藻提取物（ANE）被广泛用于提高产量和品质。本研究以模式植物拟南芥为材料，研究了两种ANE生物刺激剂（ANE A和ANE B）的成分如何影响植物mRNA转录组。使用Affymetrix Ath1微阵列，检测到ANE生物刺激剂对拟南芥植物mRNA转录组的影响存在显著异质性，这些植物积累的生物量比未处理的对照显著更多。受ANE生物刺激剂失调的基因与广泛的预测生物学过程、分子功能和亚细胞分布相关。ANE A使4.47%的转录组失调，而ANE B使0.87%的转录组失调。两种ANE的成分显著不同，多酚水平相差4倍，这是观察到的最大差异。ANE生物刺激剂成分的标准化对于对植物基因表达和生物刺激提供一致的效果而言是一项挑战。
