Sarfraz Muhammad, Shi Wenjun, Gao Yuan, Clas Sophie-Dorothee, Roa Wilson, Bou-Chacra Nadia, Löbenberg Raimar
Division of Pharmaceutical Sciences, Faculty of Pharmacy & Pharmaceutical Sciences, Katz Group-Rexall Centre for Pharmacy & Health Research, University of Alberta 11361 - 87 Avenue, Room 3-142-K Edmonton, AB T6G 2E1, Canada.
Department of Pathogenic Biology, School of Medicine, University of Tongji Shanghai, China.
Ther Deliv. 2016;7(4):213-28. doi: 10.4155/tde-2015-0007.
Secondary toxicity of nanoparticles (NPs) in macrophages is a well-known phenomenon. The aim of the study was to investigate the immuneresponse of macrophages after NP treatment.
METHODS & RESULTS: Antituberculosis drugs moxifloxacin and rifampicin were loaded into gelatin and polyisobutyl-cyanoacrylate NPs. The NPs were physicochemical characterized. Cellular immuneresponses and cellular viability were determined. The drug release kinetics vary depending on the type of NP, size and loading capacity. IC50 values of polyisobutyl-cyanoacrylate NPs were lower than for gelatin NPs. NPs treatment induced higher release of Th1 type cytokines compared with free drug.
NPs together with chemotherapeutic drugs might be able to trigger an immune response in macrophages. The combined effect might be able to overcome mycobacteria infections.
纳米颗粒（NPs）在巨噬细胞中的二次毒性是一种众所周知的现象。本研究的目的是调查纳米颗粒处理后巨噬细胞的免疫反应。
将抗结核药物莫西沙星和利福平负载到明胶和聚异丁基氰基丙烯酸酯纳米颗粒中。对纳米颗粒进行了物理化学表征。测定了细胞免疫反应和细胞活力。药物释放动力学因纳米颗粒的类型、大小和负载能力而异。聚异丁基氰基丙烯酸酯纳米颗粒的IC50值低于明胶纳米颗粒。与游离药物相比，纳米颗粒处理诱导了更高水平的Th1型细胞因子释放。
纳米颗粒与化疗药物一起可能能够在巨噬细胞中引发免疫反应。这种联合效应可能能够克服分枝杆菌感染。
