Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada.
Biomed Res Int. 2013;2013:136590. doi: 10.1155/2013/136590. Epub 2013 Jul 10.
The aim of this study was to assess the in vitro release kinetics of antituberculosis drug-loaded nanoparticles (NPs) using a "modified" cylindrical apparatus fitted with a regenerated cellulose membrane attached to a standard dissolution apparatus (modifiedcylinder method). The model drugs that were used were rifampicin (RIF) and moxifloxacin hydrochloride (MX). Gelatin and polybutyl cyanoacrylate (PBCA) NPs were evaluated as the nanocarriers, respectively. The dissolution and release kinetics of the drugs from loaded NPs were studied in different media using the modified cylinder method and dialysis bag technique was used as the control technique. The results showed that use of the modified cylinder method resulted in different release profiles associated with unique release mechanisms for the nanocarrier systems investigated. The modified cylinder method also permitted discrimination between forced and normal in vitro release of the model drugs from gelatin NPs in the presence or absence of enzymatic degradation. The use of dialysis bag technique resulted in an inability to differentiate between the mechanisms of drug release from the NPs in these cases. This approach offers an effective tool to investigate in vitro release of RIF and MX from NPs, which further indicate that this technique can be used for performance testing of nanosized carrier systems.
本研究旨在使用“改良”的圆柱形装置评估载抗结核药物纳米颗粒（NPs）的体外释放动力学，该装置配备了附着在标准溶解装置上的再生纤维素膜（改良圆柱法）。所用的模型药物为利福平（RIF）和盐酸莫西沙星（MX）。明胶和聚氰基丙烯酸正丁酯（PBCA） NPs 分别作为纳米载体进行评估。使用改良圆柱法和透析袋技术在不同介质中研究了载药 NPs 的溶解和释放动力学，透析袋技术作为对照技术。结果表明，改良圆柱法导致与所研究的纳米载体系统独特的释放机制相关的不同释放曲线。改良圆柱法还允许区分在存在或不存在酶降解的情况下明胶 NPs 中模型药物的强制和正常体外释放。在这些情况下，使用透析袋技术导致无法区分药物从 NPs 中的释放机制。该方法提供了一种有效的工具来研究 RIF 和 MX 从 NPs 中的体外释放，这进一步表明该技术可用于纳米载体系统的性能测试。
