Zhang Yu-xiu, Ma Xu, Liao Xiao-yong, Yan Xiu-lan, Ma Dong, Gong Xue-gang
Huan Jing Ke Xue. 2015 Dec;36(12):4623-9.
Pteris vittata L. can absorb and accumulate high arsenic levels in soil. To clarify the occurrence characteristics of pyrene (PYR) and arsenate (As) as well as their interaction in P. vittata L., the hosting and distribution rules of PYR were determined via two-photon laser scanning confocal microscopy (TPLSCM). The results showed that PYR addition resulted in obviously lower concentrations of total As in various parts of P. vittata, with a largest decrease of about 35% in the leaves and stem, and 20. 5% in the roots. PYR addition could also decrease the proportion of trivalent arsenic and increased that of pentavalent arsenate in different parts of P. vittata. The concentrations of trivalent and pentavalent arsenic in the leaves of P. vittata showed the largest decrement, which were 42.2% and 32.49%, respectively. Arsenate addition increased the accumulation of PYR in the root and stem of P. vittata by 9.8 µg and 139 ng per plant, respectively, while no obvious influence was found on the PYR in the leaves. Pyrene mainly attached to the cell membrane and other membrane structure such as nuclear membrane and organelle membrane, and there was less pyrene in the cytoplasm. There was little PYR in the phloem and cortex in the stem as well as palisade tissue and spongy tissue in leaves.
蜈蚣草能吸收并积累土壤中高含量的砷。为阐明芘（PYR）和砷酸盐（As）在蜈蚣草中的存在特征及其相互作用，通过双光子激光扫描共聚焦显微镜（TPLSCM）测定了PYR的寄主和分布规律。结果表明，添加PYR导致蜈蚣草各部位总砷浓度明显降低，叶片和茎中最大降幅约为35%，根中为20.5%。添加PYR还可降低蜈蚣草不同部位三价砷的比例，增加五价砷酸盐的比例。蜈蚣草叶片中三价和五价砷的浓度降幅最大，分别为42.2%和32.49%。添加砷酸盐使蜈蚣草根和茎中PYR的积累量分别增加9.8 μg/株和139 ng/株，而对叶片中的PYR无明显影响。芘主要附着在细胞膜以及核膜和细胞器膜等其他膜结构上，细胞质中芘较少。茎中的韧皮部和皮层以及叶片中的栅栏组织和海绵组织中几乎没有PYR。
