Beijing Key Laboratory of Environmental Damage Assessment and Remediation, Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences (CAS), Beijing, 100101, People's Republic of China.
Environ Sci Pollut Res Int. 2017 Nov;24(33):26136-26141. doi: 10.1007/s11356-017-0419-y. Epub 2017 Oct 12.
In order to illuminate the mechanism of the interaction of polycyclic aromatic hydrocarbon (PAH) with different benzene rings and arsenic (As) in As hyperaccumulator, Pteris vittata L., the uptakes of PAHs were investigated using hydroponics simulation and localizations of PAHs in the plant were determined using two-photon laser scanning confocal microscopy (TPLSCM). The results showed that the total As concentration in different parts of P. vittata decreased in the presence of PAHs with increased numbers of benzene rings: 38.0-47.4% for benzo(a)pyrene (BaP, five rings), 20.5-35.9% for pyrene (PYR, four rings), and 13.7-16.6% for fluorine (FLU, three rings). BaP and PYR concentrations increased, while FLU concentration decreased in the presence of As. The results of TPLSCM revealed that PAHs distributed in epidermal cells of roots, xylem, and endothelial cells of rachis, epidermis, and stomatal cells of pinnae; however, the fluorescence intensity of BaP and PYR were higher than FLU significantly in plant. This study provided important basis to further research on interactive effects of PAHs and As in the P. vittata. These findings were important to understand the mechanisms of PAH and As translocation and distribution by P. vittata.
为了阐明多环芳烃（PAH）与不同苯环和砷（As）在砷超积累植物蜈蚣草中的相互作用机制，采用水培模拟法研究了 PAHs 的吸收，并用双光子激光扫描共聚焦显微镜（TPLSCM）确定了 PAHs 在植物中的定位。结果表明，随着苯环数量的增加，不同部位的蜈蚣草总砷浓度降低：苯并（a）芘（BaP，五环）为 38.0-47.4%，芘（PYR，四环）为 20.5-35.9%，芴（FLU，三环）为 13.7-16.6%。在砷存在的情况下，BaP 和 PYR 浓度增加，而 FLU 浓度降低。TPLSCM 的结果表明，PAHs 分布在根表皮细胞、木质部、叶脉内皮细胞、羽片表皮和气孔细胞中；然而，BaP 和 PYR 的荧光强度明显高于 FLU。本研究为进一步研究蜈蚣草中 PAHs 和 As 的相互作用提供了重要依据。这些发现对于理解蜈蚣草中 PAH 和 As 的转运和分布机制具有重要意义。
