Li Hu, Huang Fu-yi, Su Jian-qiang, Hong You-wei, Yu Shen
Huan Jing Ke Xue. 2015 Dec;36(12):4659-66.
Ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) play important roles in the biogeochemical nitrogen cycle. Rivers are important ecosystems containing a large number of functional microbes in nitrogen cycle. In this study, denaturing gradient gel electrophoresis (DGGE ) and real-time quantitative PCR (qPCR) technology were used to analyze the distribution and diversity of AOA and AOB in sediments from Oujiang. The results showed that the AOA community structure was similar among various sites, while the AOB community structure was significantly different, in which all detected AOB sequences were classified into Nitrosospira and Nitrosomonas, and 90% affiliated to Nitrosospira. The community composition of AOA was influenced by NH₄⁺ and TS, in addition, the AOB composition was affected by NH₄⁺, EC, pH, NO₃⁻, TC and TN. Total sulfur (TS) and electrical conductivity (EC) were the major factors influencing the diversity of AOA and AOB, respectively. AOA abundance was significantly higher than that of AOB. EC, NH₄⁺-N and NO₃⁻-N were the main environmental factors affecting the abundance of AOA and AOB. This study indicated that the community composition and diversity of AOA and AOB were significantly influenced by environmental factors, and AOA might be dominant drivers in the ammonia oxidation process in Oujiang surface sediment.
氨氧化古菌（AOA）和氨氧化细菌（AOB）在生物地球化学氮循环中发挥着重要作用。河流是重要的生态系统，在氮循环中含有大量功能微生物。本研究采用变性梯度凝胶电泳（DGGE）和实时定量PCR（qPCR）技术分析了瓯江沉积物中AOA和AOB的分布及多样性。结果表明，不同位点的AOA群落结构相似，而AOB群落结构差异显著，所有检测到的AOB序列均归为亚硝化螺菌属和亚硝化单胞菌属，其中90%隶属于亚硝化螺菌属。AOA的群落组成受NH₄⁺和总硫（TS）影响，此外，AOB组成受NH₄⁺、电导率（EC）、pH、NO₃⁻、总碳（TC）和总氮（TN）影响。总硫（TS）和电导率（EC）分别是影响AOA和AOB多样性的主要因素。AOA丰度显著高于AOB。EC、NH₄⁺-N和NO₃⁻-N是影响AOA和AOB丰度的主要环境因素。本研究表明，AOA和AOB的群落组成及多样性受环境因素显著影响，且AOA可能是瓯江表层沉积物氨氧化过程中的主要驱动因素。
