Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Allahabad 211004, U.P., India.
Bioenergy and Energy Planning Research Group, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.
Bioresour Technol. 2016 Aug;213:262-269. doi: 10.1016/j.biortech.2016.02.091. Epub 2016 Mar 9.
In this study Bacillus sp. MTCC5877 was explored for the production of biosurfactant (BSs) and various carbon sources 1% (w/v), 0.5% (w/v) nitrogen sources were tested at different pH, and temperature. Yield was measured in terms of Emulsification index (EI), Oil Displacement Area (ODA) and Drop Collapse Area (DCA) and maximum emulsification activities of BSs were found (E24) 50%, 76% and 46%, respectively, and maximum ODA of 5.0, 6.2 and 4.7cm, were shown respectively. The BS was able to reduce the surface tension of water from 72 to 30mN/m and 72 to 32mN/m. Structural compositions of BS were confirmed by FTIR, GC-MS and NMR. Anti-adhesive property of BS was determined and found effective against biofilm formation. It could remove 73% Cd from vegetable which confirms its application in food industry.
在这项研究中，探索了芽孢杆菌 MTCC5877 产生生物表面活性剂（BSs）的能力，并测试了不同 pH 值和温度下 1%（w/v）和 0.5%（w/v）的各种碳源和氮源。产量以乳化指数（EI）、油置换面积（ODA）和液滴塌陷面积（DCA）来衡量，BS 的最大乳化活性分别为 50%、76%和 46%，最大 ODA 分别为 5.0、6.2 和 4.7cm。BS 能够将水的表面张力从 72 降低到 30mN/m 和 72 降低到 32mN/m。通过傅里叶变换红外光谱（FTIR）、气相色谱-质谱联用（GC-MS）和核磁共振（NMR）确认了 BS 的结构组成。测定了 BS 的抗粘附性能，发现其对生物膜形成有效。它可以从蔬菜中去除 73%的镉，这证实了它在食品工业中的应用。
