野甜瓜亚种野甜瓜改善叙利亚金仓鼠高脂饮食诱导的血脂异常并抑制3T3-L1脂肪细胞的脂肪生成。

Cucumis melo ssp. Agrestis var. Agrestis Ameliorates High Fat Diet Induced Dyslipidemia in Syrian Golden Hamsters and Inhibits Adipogenesis in 3T3-L1 Adipocytes.

作者信息

Shankar Kripa, Singh Sumit K, Kumar Durgesh, Varshney Salil, Gupta Abhishek, Rajan Sujith, Srivastava Ankita, Beg Muheeb, Srivastava Anurag Kumar, Kanojiya Sanjeev, Mishra Dipak K, Gaikwad Anil N

机构信息

Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India.

Division of Botany, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India.

出版信息

Pharmacogn Mag. 2015 Oct;11(Suppl 4):S501-10. doi: 10.4103/0973-1296.172945.

DOI:10.4103/0973-1296.172945

PMID:27013786

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4787080/

Abstract

BACKGROUND

Cucumis melo ssp. agrestis var. agrestis (CMA) is a wild variety of C. melo. This study aimed to explore anti-dyslipidemic and anti-adipogenic potential of CMA.

MATERIALS AND METHODS

For initial anti-dyslipidemic and antihyperglycemic potential of CMA fruit extract (CMFE), male Syrian golden hamsters were fed a chow or high-fat diet with or without CMFE (100 mg/kg). Further, we did fractionation of this CMFE into two fractions namely; CMA water fraction (CMWF) and CMA hexane fraction (CMHF). Phytochemical screening was done with liquid chromatography-mass spectrometry LC- (MS)/MS and direct analysis in real time-MS to detect active compounds in the fractions. Further, high-fat diet fed dyslipidemic hamsters were treated with CMWF and CMHF at 50 mg/kg for 7 days.

RESULTS

Oral administration of CMFE and both fractions (CMWF and CMHF) reduced the total cholesterol, triglycerides, low-density lipoprotein cholesterol, and very low-density lipoprotein-cholesterol levels in high fat diet-fed dyslipidemic hamsters. CMHF also modulated expression of genes involved in lipogenesis, lipid metabolism, and reverse cholesterol transport. Standard biochemical diagnostic tests suggested that neither of fractions causes any toxicity to hamster liver or kidneys. CMFE and CMHF also decreased oil-red-O accumulation in 3T3-L1 adipocytes.

CONCLUSION

Based on these results, it is concluded that CMA possesses anti-dyslipidemic and anti-hyperglycemic activity along with the anti-adipogenic activity.

SUMMARY

The oral administration of Cucumis melo agrestis fruit extract (CMFE) and its fractions (CMWF and CMHF) improved serum lipid profile in HFD fed dyslipidemic hamsters.CMFE, CMWF and CMHF significantly attenuated body weight gain and eWAT hypertrophy.The CMHF decreased lipogenesis in both liver and adipose tissue.CMFE and CMHF also inhibited adipogenesis in 3T3-L1 adipocytes. Abbreviation used: CMA: Cucumis melo ssp. agrestis var. agrestis, CMFE: CMA fruit extract, CMWF: CMA water fraction, CMHF: CMA hexane fraction, FAS: Fatty acid synthase, SREBP1c: Sterol regulatory element binding protein 1c, ACC: Acetyl CoA carboxylase, LXR α: Liver X receptor α.

摘要

背景

野甜瓜（Cucumis melo ssp. agrestis var. agrestis，CMA）是甜瓜的一个野生变种。本研究旨在探究CMA的抗血脂异常和抗脂肪生成潜力。

材料与方法

为了初步评估CMA果实提取物（CMFE）的抗血脂异常和降血糖潜力，将雄性叙利亚金仓鼠分为正常饮食组或高脂饮食组，高脂饮食组又分为添加或不添加CMFE（100毫克/千克）的两组。此外，我们将该CMFE分离为两个组分，即CMA水相组分（CMWF）和CMA己烷相组分（CMHF）。采用液相色谱 - 质谱联用（LC - MS）/ MS和实时直接分析质谱法进行植物化学筛选，以检测各组分中的活性化合物。进一步地，对高脂饮食诱导的血脂异常仓鼠以50毫克/千克的剂量给予CMWF和CMHF处理7天。

结果

口服CMFE以及两个组分（CMWF和CMHF）均可降低高脂饮食喂养的血脂异常仓鼠的总胆固醇、甘油三酯、低密度脂蛋白胆固醇和极低密度脂蛋白胆固醇水平。CMHF还调节了参与脂肪生成、脂质代谢和胆固醇逆向转运的基因表达。标准生化诊断测试表明，两个组分均未对仓鼠肝脏或肾脏造成任何毒性。CMFE和CMHF还减少了3T3 - L1脂肪细胞中油红O的积累。

结论

基于这些结果，得出结论：CMA具有抗血脂异常、降血糖活性以及抗脂肪生成活性。

总结

口服野甜瓜果实提取物（CMFE）及其组分（CMWF和CMHF）可改善高脂饮食喂养的血脂异常仓鼠的血脂谱。CMFE、CMWF和CMHF显著减轻体重增加和附睾白色脂肪组织肥大。CMHF降低肝脏和脂肪组织中的脂肪生成。CMFE和CMHF还抑制3T3 - L1脂肪细胞的脂肪生成。使用的缩写：CMA：野甜瓜（Cucumis melo ssp. agrestis var. agrestis），CMFE：CMA果实提取物，CMWF：CMA水相组分，CMHF：CMA己烷相组分，FAS：脂肪酸合酶，SREBP1c：固醇调节元件结合蛋白1c，ACC：乙酰辅酶A羧化酶，LXRα：肝脏X受体α 。