Lekka Małgorzata
Institute of Nuclear Physics, PAS, Radzikowskiego 152, 31-342 Kraków, Poland.
Bionanoscience. 2016;6:65-80. doi: 10.1007/s12668-016-0191-3. Epub 2016 Jan 30.
Currently, biomechanics of living cells is in the focus of interest due to noticeable capability of such techniques like atomic force microscopy (AFM) to probe cellular properties at the single cell level directly on living cells. The research carried out, so far, delivered data showing, on the one hand, the use of cellular mechanics as a biomarker of various pathological changes, which, on the other hand, reveal relative nature of biomechanics. In the AFM, the elastic properties of living cells are delivered from indentation experiments and described quantitatively by Young's modulus defined here as a measure of cellular deformability. Here, the AFM studies directly comparing the mechanical properties of normal and cancerous cells are summarized and presented together with a few important issues related to the relativeness of Young's modulus.
目前，活细胞生物力学成为研究热点，这是因为诸如原子力显微镜（AFM）等技术具有显著能力，能够在活细胞上直接于单细胞水平探测细胞特性。迄今为止所开展的研究提供的数据表明，一方面，细胞力学可作为各种病理变化的生物标志物，另一方面，这些数据也揭示了生物力学的相对性质。在原子力显微镜中，活细胞的弹性特性通过压痕实验得出，并由杨氏模量进行定量描述，在此将杨氏模量定义为细胞可变形性的一种度量。本文总结了直接比较正常细胞和癌细胞力学特性的原子力显微镜研究，并阐述了与杨氏模量相对性相关的一些重要问题。
