Mahdavi Mehdi, Ebtekar Massoumeh, Hassan Zuhair Mohammad, Faezi Sobhan, Khorram Khorshid Hamidreza, Taghizadeh Morteza, Azadmanesh Keyhan
Department of Immunology, Pasteur Institute of Iran, Tehran, Iran.
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Int J Mol Cell Med. 2015 Fall;4(4):218-26.
Memory formation is the most important aspect of a vaccine which can guarantee long-lasting immunity and protection. The main aim of the present study was to evaluate the memory immune responses after immunization with a mini vaccine. Mice were immunized with human immunodeficiency virus-1 P24-Nef fusion peptide and then cellular and humoral immune responses were evaluated. In order to determine long-lived memory, immune responses were monitored for 20 weeks after final immunization. The results showed that the candidate vaccine induced proliferation and cytotoxic T lymphocyte responses and shifted cytokine patterns to T helper-1 profile. Evaluation of humoral immune responses also showed an increase in total peptide specific-IgG titer and a shift to IgG2a humoral response. Monitoring of immune responses at weeks 4, 12 and 20 after last immunization showed that immunologic parameters have been sustained for 20 weeks. Our findings support the notion that long-lived memory responses were achieved using a mini vaccine immunization.
记忆形成是疫苗最重要的方面，它能够保证持久的免疫力和保护作用。本研究的主要目的是评估用微型疫苗免疫后的记忆免疫反应。用人类免疫缺陷病毒1型P24-Nef融合肽对小鼠进行免疫，然后评估细胞免疫和体液免疫反应。为了确定长期记忆，在末次免疫后20周监测免疫反应。结果表明，候选疫苗诱导了增殖和细胞毒性T淋巴细胞反应，并使细胞因子模式向辅助性T细胞1型转变。体液免疫反应评估还显示，总肽特异性IgG滴度增加，且体液反应向IgG2a转变。在末次免疫后第4、12和20周监测免疫反应，结果显示免疫参数持续了20周。我们的研究结果支持了这样一种观点，即使用微型疫苗免疫可实现长期记忆反应。
