幽门螺杆菌FlgD的稳定性数据及其与其他同源物的结构比较。

Stability data of FlgD from Helicobacter pylori and structural comparison with other homologs.

作者信息

Pulić Ivana, Cendron Laura, Salamina Marco, Polverino de Laureto Patrizia, Matković-Čalogović Dubravka, Zanotti Giuseppe

机构信息

University of Zagreb, Faculty of Science, Department of Chemistry, Division of General and Inorganic Chemistry, Horvatovac 102a, Zagreb 10000, Croatia; Department of Biomedical Sciences, University of Padua, Via Ugo Bassi 58/B, Padua 35131, Italy.

Department of Biology, University of Padua, Via Ugo Bassi 58/B, Padua 35131, Italy.

出版信息

Data Brief. 2016 Mar 4;7:493-501. doi: 10.1016/j.dib.2016.02.068. eCollection 2016 Jun.

DOI:10.1016/j.dib.2016.02.068

PMID:27014738

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4792863/

Abstract

Flagellin component D (FlgD) from Helicobacter pylori is involved in the assembly of the hook of flagella, helical tubular structures that provide motility in non-filamentous bacteria. Data provided in this article refer to HpFlgD from strains 26695 (HpFlgD_26695) and G27 (HpFlgD_G27). Within this article, information on the secondary structure content and different type of interfaces found in the two crystal forms of HpFlgD (monoclinic, HpFlgD_m and tetragonal, HpFlgD_t) are provided, as well as the list of the hydrogen bonds between monomers that are relevant for their assembly into a tetramer. Additionally, data involving investigation of the size of HpFlgD in the solution and the crystallized HpFlgD are presented, "Crystal structure of truncated FlgD from the human pathogen Helicobacter pylori" [1]. The superposition of the different domains of HpFlgD (Fn-III and tudor domains) with the similar domains found in other species is shown, as well as the superposition of HpFlgD and modeled HpFlgE (flagellar hook protein).

摘要

幽门螺杆菌的鞭毛蛋白组分D（FlgD）参与鞭毛钩的组装，鞭毛钩是一种螺旋管状结构，可赋予非丝状细菌运动能力。本文提供的数据涉及26695菌株（HpFlgD_26695）和G27菌株（HpFlgD_G27）的HpFlgD。本文提供了关于HpFlgD两种晶体形式（单斜晶系，HpFlgD_m和四方晶系，HpFlgD_t）的二级结构含量和不同类型界面的信息，以及单体之间与它们组装成四聚体相关的氢键列表。此外，还展示了涉及溶液中HpFlgD大小和结晶HpFlgD的研究数据，“人类病原体幽门螺杆菌截短型FlgD的晶体结构”[1]。展示了HpFlgD不同结构域（Fn-III和tudor结构域）与其他物种中相似结构域的叠加，以及HpFlgD与模拟的HpFlgE（鞭毛钩蛋白）的叠加。