Attar Mohammad Mahdi, Haghpanahi Mohammad
a Department of Mechanical and Aerospace Engineering, Science and Research Branch , Islamic Azad University , Tehran , Iran.
b Department of Mechanical Engineering , Iran University of Science and Technology , Tehran , Iran.
Electromagn Biol Med. 2016;35(4):305-20. doi: 10.3109/15368378.2015.1089409. Epub 2016 Mar 25.
The purpose of this study was to propose a method for constructing the software setup required for investigating thermal effect of superparamagnetic nanoparticles on three human cell lines. This article aimed to examine the required nanoparticle dose, frequency, field intensity and the exposure time.
In the present study, first some general details were given about design and construction of the setup required for generating a safe magnetic field in order to examine the thermal effect of superparamagnetic nanoparticles on three human cancer cell lines, cultured under laboratory conditions. Next, a series of experimental tests were conducted to study the effect of magnetic field, on the cells. Finally, by applying three types of iron-based nanoparticles with mean diameters of 8, 15 and 20 nm, for 30 min, the temperature rise and specific absorption rate (SAR) were calculated.
By conducting experimental tests, the maximum temperature rise at the resonance frequency of the coil was reported to be 80 kHz, and it was observed that all the cells died when temperature of the cells reached 42°C/30 min. Based on the experiments, it was observed that magnetic field with intensity of 8 kA/m within the frequency range of 80-180 kHz did not have any effect on the cells.
Based on the results, it can be concluded that the nanoparticle dose of 80 µg/ml with diameter of 8 nm at the resonance frequency of coil for 30 min was sufficient to destroy all the cancerous cells in the flask.
本研究的目的是提出一种构建软件设置的方法，用于研究超顺磁性纳米颗粒对三种人类细胞系的热效应。本文旨在研究所需的纳米颗粒剂量、频率、场强和暴露时间。
在本研究中，首先给出了一些关于为研究超顺磁性纳米颗粒对在实验室条件下培养的三种人类癌细胞系的热效应而产生安全磁场所需设置的设计和构建的一般细节。接下来，进行了一系列实验测试以研究磁场对细胞的影响。最后，通过应用三种平均直径为8、15和20nm的铁基纳米颗粒30分钟，计算了温度升高和比吸收率（SAR）。
通过进行实验测试，据报道线圈共振频率下的最大温度升高为80kHz，并且观察到当细胞温度达到42°C/30分钟时所有细胞均死亡。基于实验，观察到在80-180kHz频率范围内强度为8kA/m的磁场对细胞没有任何影响。
基于结果可以得出结论，在80μg/ml的纳米颗粒剂量、8nm的直径、线圈共振频率下作用30分钟足以破坏培养瓶中的所有癌细胞。
