Wang Qian, Subramanian Palaniappan, Schechter Alex, Teblum Eti, Yemini Reut, Nessim Gilbert Daniel, Vasilescu Alina, Li Musen, Boukherroub Rabah, Szunerits Sabine
Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University , Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.
Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education), Shandong University , Jinan 250061, China.
ACS Appl Mater Interfaces. 2016 Apr 20;8(15):9600-9. doi: 10.1021/acsami.6b00663. Epub 2016 Apr 5.
The number of patients suffering from inflammatory bowel disease (IBD) is increasing worldwide. The development of noninvasive tests that are rapid, sensitive, specific, and simple would allow preventing patient discomfort, delay in diagnosis, and the follow-up of the status of the disease. Herein, we show the interest of vertically aligned nitrogen-doped carbon nanotube (VA-NCNT) electrodes for the required sensitive electrochemical detection of lysozyme in serum, a protein that is up-regulated in IBD. To achieve selective lysozyme detection, biotinylated lysozyme aptamers were covalently immobilized onto the VA-NCNTs. Detection of lysozyme in serum was achieved by measuring the decrease in the peak current of the Fe(CN)6(3-/4-) redox couple by differential pulse voltammetry upon addition of the analyte. We achieved a detection limit as low as 100 fM with a linear range up to 7 pM, in line with the required demands for the determination of lysozyme level in patients suffering from IBD. We attained the sensitive detection of biomarkers in clinical samples of healthy patients and individuals suffering from IBD and compared the results to a classical turbidimetric assay. The results clearly indicate that the newly developed sensor allows for a reliable and efficient analysis of lysozyme in serum.
全球范围内，炎症性肠病（IBD）患者数量正在增加。开发快速、灵敏、特异且简便的非侵入性检测方法，将有助于避免患者不适、诊断延迟以及疾病状态的随访。在此，我们展示了垂直排列的氮掺杂碳纳米管（VA-NCNT）电极对于血清中溶菌酶所需灵敏电化学检测的优势，溶菌酶是一种在IBD中上调的蛋白质。为实现溶菌酶的选择性检测，将生物素化的溶菌酶适配体共价固定在VA-NCNT上。通过差分脉冲伏安法测量加入分析物后Fe(CN)6(3-/4-)氧化还原对的峰值电流降低，实现血清中溶菌酶的检测。我们实现了低至100 fM的检测限和高达7 pM的线性范围，符合IBD患者溶菌酶水平测定的要求。我们在健康患者和IBD患者的临床样本中实现了生物标志物的灵敏检测，并将结果与经典比浊法进行了比较。结果清楚地表明，新开发的传感器能够对血清中的溶菌酶进行可靠且高效的分析。
