用于酵母复制寿命研究的微流控技术
Microfluidic technologies for yeast replicative lifespan studies.
作者信息
Chen Kenneth L, Crane Matthew M, Kaeberlein Matt
机构信息
Department of Pathology, University of Washington, Seattle, WA, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA; Medical Scientist Training Program, University of Washington, Seattle, WA, USA.
Department of Pathology, University of Washington, Seattle, WA, USA.
出版信息
Mech Ageing Dev. 2017 Jan;161(Pt B):262-269. doi: 10.1016/j.mad.2016.03.009. Epub 2016 Mar 23.
Abstract
The budding yeast Saccharomyces cerevisiae has been used as a model organism for the study of aging for over 50 years. In this time, the canonical aging experiment-replicative lifespan analysis by manual microdissection-has remained essentially unchanged. Recently, microfluidic technologies have been developed that may be able to substitute for this time- and labor-intensive procedure. These technologies also allow cell physiology to be observed throughout the entire lifetime. Here, we review these devices, novel observations they have made possible, and some of the current system limitations.
摘要
出芽酵母酿酒酵母已被用作研究衰老的模式生物超过50年。在此期间，经典的衰老实验——通过手动显微操作进行的复制寿命分析——基本保持不变。最近，微流控技术已经得到发展，可能能够替代这种耗时且费力的程序。这些技术还能在细胞的整个生命周期内观察细胞生理学。在这里，我们回顾这些设备、它们所带来的新观察结果以及当前一些系统局限性。
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