使用 HYAA 微流控平台测量酿酒酵母的复制寿命。
Measuring the Replicative Lifespan of Saccharomyces cerevisiae Using the HYAA Microfluidic Platform.
机构信息
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Huffington Center on Aging, Baylor College of Medicine, Houston, TX, USA.
出版信息
Methods Mol Biol. 2020;2144:1-6. doi: 10.1007/978-1-0716-0592-9_1.
The replicative aging of the budding yeast, Saccharomyces cerevisiae, has been a useful model for dissecting the molecular mechanisms of the aging process. Traditionally, the replicative lifespan (RLS) is measured by manually dissecting mother cells from daughter cells, which is a very tedious process. Since 2012, several microfluidic systems have been developed to automate the dissection process, significantly accelerating RLS determination. Here, we describe a detailed protocol of RLS measurement using a ommercially available microfluidic system based on the HYAA chip design, which enables data collection of up to 8000 cells in a single experiment.
酿酒酵母的复制性衰老一直是解析衰老过程分子机制的有用模型。传统上，通过手动从子细胞中分离母细胞来测量复制寿命（RLS），这是一个非常繁琐的过程。自 2012 年以来，已经开发了几种微流控系统来自动化分离过程，显著加快了 RLS 的确定速度。在这里，我们描述了一种使用基于 HYAA 芯片设计的商业微流控系统进行 RLS 测量的详细方案，该系统可在单个实验中收集多达 8000 个细胞的数据。
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