Fourie Arista, Wingfield Michael J, Wingfield Brenda D, Thu Pham Q, Barnes Irene
Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0002, South Africa.
Forest Protection Research Centre, Vietnamese Academy of Forest Sciences, Dong Ngac, Tu Liem, Hanoi, Vietnam.
Infect Genet Evol. 2016 Jul;41:73-83. doi: 10.1016/j.meegid.2016.03.011. Epub 2016 Mar 23.
The fungal pathogen, Ceratocystis manginecans, has caused serious canker and wilt disease on Mangifera indica (mango), on legume tree species in Oman and Pakistan and on Acacia spp. in Indonesia. A Ceratocystis species, with similar morphology to C. manginecans, has recently been reported in Vietnam, causing severe disease of Acacia trees. Previous population genetic studies on isolates from M. indica in Oman and Pakistan have shown that the pathogen represents a single clonal haplotype, indicative of an introduced pathogen. The aim of this study was to investigate the genetic diversity and population structure of 160 C. manginecans isolates, from four host-associated populations from Oman, Pakistan, Indonesia and Vietnam. This was done by applying a combination of 14 previously developed microsatellite markers and a new set, designed in this study from two different C. manginecans genomes. Sequence data confirmed that the isolates in Vietnam are the same species as those in Indonesia and were thus identified as C. manginecans. Unlike the populations in Oman and Pakistan, relatively high levels of genetic variation were found for the isolates from Indonesia and Vietnam. The Vietnam population was significantly differentiated from the other populations and isolates from this area had the highest level of genetic diversity thus far encountered for the pathogen.
真菌病原体芒果囊孢壳菌（Ceratocystis manginecans）已在芒果（Mangifera indica）、阿曼和巴基斯坦的豆科树种以及印度尼西亚的金合欢属植物上引发了严重的溃疡病和枯萎病。最近在越南报道了一种形态与芒果囊孢壳菌相似的囊孢壳菌物种，它导致了金合欢树的严重病害。此前对阿曼和巴基斯坦芒果树上分离菌株的群体遗传学研究表明，该病原体代表单一的克隆单倍型，表明它是一种外来病原体。本研究的目的是调查来自阿曼、巴基斯坦、印度尼西亚和越南四个宿主相关群体的160株芒果囊孢壳菌分离株的遗传多样性和群体结构。这是通过应用14个先前开发的微卫星标记以及本研究从两个不同的芒果囊孢壳菌基因组设计的一组新标记来完成的。序列数据证实越南的分离株与印度尼西亚的分离株为同一物种，因此被鉴定为芒果囊孢壳菌。与阿曼和巴基斯坦的群体不同，印度尼西亚和越南的分离株发现了相对较高水平的遗传变异。越南群体与其他群体有显著差异，该地区的分离株具有该病原体迄今为止所遇到的最高水平的遗传多样性。
