Liu Feifei, Duong Tuan A, Barnes Irene, Wingfield Michael J, Chen Shuaifei
China Eucalypt Research Centre (CERC), Chinese Academy of Forestry (CAF), Zhanjiang 524022, China.
Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0028, South Africa.
J Fungi (Basel). 2021 Sep 15;7(9):759. doi: 10.3390/jof7090759.
Species in the complex are important fungal pathogens of plantation trees globally. The most important hosts include species of , , , and . Despite their relevance and widespread occurrence, little is known regarding their population genetics and how this might relate to their host associations or geographic regions in which they occur. A global collection of 491 isolates representing the complex, from four different plant hosts and nine countries, were genotyped using microsatellite markers. Population genetic analyses using numerous tools were conducted to interrogate how their genetic diversity and structure might be affected by host or areas of occurrence. Results of genetic diversity studies showed that when grouping isolates into populations based on their host associations, the population on was most diverse, and it also has a broad global distribution. When considering countries of origin as a basis for defining populations, the gene and genotypic diversity were highest in populations from China, Indonesia, and Brazil. In contrast, populations from Oman and Pakistan collected from had the lowest genetic diversity and were clonal. Molecular variance, population differentiation, and network and structure analyses showed that the genetic structure of isolates in the complex is influenced by both host association as well as geographical isolation. Furthermore, the results reflected the movement of genotypes between plant hosts and geographic regions that have implications regarding the broad global distribution of this pathogen.
该复合种中的物种是全球范围内人工林树木重要的真菌病原体。最重要的寄主包括[寄主1物种名]、[寄主2物种名]、[寄主3物种名]和[寄主4物种名]的物种。尽管它们具有重要意义且广泛存在，但对于它们的种群遗传学以及这如何与其寄主关联或它们所出现的地理区域相关，人们了解甚少。使用微卫星标记对来自四个不同植物寄主和九个国家的代表该复合种的491个分离株进行了全球收集并进行基因分型。使用多种工具进行了种群遗传分析，以探究它们的遗传多样性和结构如何受到寄主或出现区域的影响。遗传多样性研究结果表明，当根据寄主关联将分离株分组为种群时，[寄主1]上的种群最为多样，并且它也具有广泛的全球分布。当以原产国为基础定义种群时，来自中国、印度尼西亚和巴西的种群的基因和基因型多样性最高。相比之下，从[寄主1]收集的来自阿曼和巴基斯坦的种群遗传多样性最低且为克隆型。分子方差、种群分化以及网络和结构分析表明，该复合种中分离株的遗传结构受寄主关联以及地理隔离的影响。此外，结果反映了基因型在植物寄主和地理区域之间的移动，这对该病原体的广泛全球分布具有影响。
