一种用于透射电子显微镜研究植入前人类胚胎细胞质片段的新方法。

A novel method for transmission electron microscopy study of cytoplasmic fragments from preimplantation human embryos.

作者信息

Halvaei Iman, Khalili Mohammad Ali, Nottola Stefania A

机构信息

Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, La Sapienza University of Rome, Rome, Italy.

出版信息

Microsc Res Tech. 2016 Jun;79(6):459-62. doi: 10.1002/jemt.22657. Epub 2016 Mar 26.

DOI:10.1002/jemt.22657

PMID:27016380

Abstract

Transmission electron microscopy (TEM) is the main tool for exploring the intracellular damage and organelle distribution. The cause of producing embryo cytoplsamic fragmentation is not completely understood. Since the fragments have detrimental effects on embryo development, the ultrastructural analysis of fragments may play an important role in fragmentation etiology and in embryo development as well. There are no studies regarding the ultrastructure of fragments in transferable embryos, because the preparation for TEM is not vital and embryos are discarded inevitably. This study aims to introduce a new method for ultrastructural evaluation of fragments without damaging the human cleaving embryos. Microsc. Res. Tech. 79:459-462, 2016. © 2016 Wiley Periodicals, Inc.

摘要

透射电子显微镜（TEM）是探索细胞内损伤和细胞器分布的主要工具。胚胎细胞质碎片化产生的原因尚未完全明确。由于这些碎片对胚胎发育有不利影响，因此对碎片进行超微结构分析可能在碎片病因学以及胚胎发育方面都发挥重要作用。目前尚无关于可移植胚胎中碎片超微结构的研究，因为透射电子显微镜样本制备并非至关重要，胚胎不可避免地会被丢弃。本研究旨在引入一种新方法，用于在不损伤人类分裂期胚胎的情况下对碎片进行超微结构评估。《显微镜研究与技术》79:459 - 462, 2016年。© 2016威利期刊公司。