Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
Water Res. 2016 May 15;95:319-29. doi: 10.1016/j.watres.2015.12.046. Epub 2016 Jan 23.
Although efficient removal of carbon (C), nitrogen (N) and phosphorous (P) from wastewater with low C/N ratio was achieved in anaerobic/aerobic simultaneous nitrification-endogenous denitrification and phosphorus removal (SNEDPR) systems, the removal pathways and metabolic transformations in this complex system are unclear. This work targeted at developing the stoichiometric models for denitrifying glycogen organisms (DGAOs) via nitrite and nitrate (DGAONi and DGAONa), and demonstrating a novel methodology to quantify diverse functional microorganisms (e.g. ammonia and nitrite oxidizing bacteria, aerobic phosphorus accumulating organisms (APAOs), denitrifying PAOs (DPAOs) and aerobic GAOs (AGAOs)) for the removal of C, N and P. The results showed that the anaerobic intracellular carbon storage (CODintra) was mainly accomplished by GAOs, and PAOs were only responsible for about 40% of CODintra through a stable P release. At the aerobic stage, 84.9% of P was removed by APAOs with 15.1% left by DPAOs, while 64.6% of N was removed by DGAOs (45.8% by DGAONi and 18.8% by DGAONa) with 18.1% by DPAOs and 17.3% by bacterial growth. High proportion of N removal via nitrite (partial nitrification-endogenous denitrification) (71%) saved 7.3% aeration and 38% intracellular carbon demand. However, AGAOs still activated well at the aerobic intercellular carbon consumption, which limited the further improvement of N removal efficiency. By elucidating the nutrient removal pathways among diverse functional microorganisms, the methodology developed in this study could accelerate the nutrient removal in the SNEDPR process.
虽然在厌氧/好氧同步硝化-内源反硝化除磷（SNEDPR）系统中可以有效地去除低碳氮比废水中的碳（C）、氮（N）和磷（P），但该复杂系统中的去除途径和代谢转化尚不清楚。本工作旨在为通过亚硝酸盐和硝酸盐进行反硝化糖原有机物（DGAONi 和 DGAONa）的计量模型开发，以及展示一种定量测定多种功能微生物（例如氨和亚硝酸盐氧化菌、好氧除磷菌（APAOs）、反硝化除磷菌（DPAOs）和好氧聚糖氧化菌（AGAOs））的新方法，用于去除 C、N 和 P。结果表明，厌氧细胞内碳储存（CODintra）主要由 GAOs 完成，而 PAOs 通过稳定的 P 释放仅负责 CODintra 的约 40%。在好氧阶段，APAOs 去除了 84.9%的 P，DPAOs 留下了 15.1%，而 DGAOs（45.8%通过 DGAONi 和 18.8%通过 DGAONa）去除了 64.6%的 N，DPAOs 去除了 18.1%，细菌生长去除了 17.3%。通过亚硝酸盐（部分硝化-内源反硝化）去除高比例的 N（71%）节省了 7.3%的曝气和 38%的细胞内碳需求。然而，AGAOs 仍然在好氧细胞间碳消耗中很好地激活，这限制了 N 去除效率的进一步提高。通过阐明不同功能微生物之间的营养去除途径，本研究中开发的方法可以加速 SNEDPR 过程中的营养去除。
