National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing, 100124, China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing, 100124, China.
Environ Res. 2022 Sep;212(Pt D):113464. doi: 10.1016/j.envres.2022.113464. Epub 2022 May 24.
The rapid start-up and advanced nutrient removal of simultaneous nitrification, endogenous denitrification, and phosphorus (P) removal aerobic granular sequence batch reactor (SNEDPR-AGSBR) is a challenge in the treatment of low carbon/nitrogen (C/N) domestic sewage. In this study, the feasibility of the SNEDPR-AGSBR process was examined in an exceedingly single-stage anaerobic/aerobic/anoxic sequencing batch reactor for treating low C/N ratio (3.3-5.0) domestic sewage. The initial results showed that accompanied by the rapid formation of the mature aerobic granular sludge based on the selection for slow-growing organisms, the rapid start-up (38 d) of the SNEDPR-AGSBR process was successfully realized. The formed mature aerobic granules had a dense structure with an average diameter of 667.7 μm and SVI of 30.0 mL/g. Two conditions for achieving the competitive balance between phosphorus-accumulating organisms/denitrifying phosphorus-accumulating organisms (PAOs/DPAOs) and glycogen accumulating organisms/denitrifying glycogen accumulating organisms (GAOs/DGAOs) were revealed by the long-term operation results. First, the dissolved oxygen (DO) concentration needed to be decreased to 3.0 mg/L in the aerobic phase, and then, the aerobic and anoxic phase hydraulic retention time (HRT) should be increased to 3.0 h. Notably, high removal efficiencies for NH-N (100%), total nitrogen (84.3%), and P (91.8%) of the SNEDPR-AGSBR process were stably obtained with a low C/N ratio of 3.9 domestic sewage. Simultaneous nitrification and endogenous denitrification (SNED) efficiency of 61.6% was achieved during a long-term operation of 142 days. Finally, microbial community analysis confirmed that GAOs (Defluviicoccus)/DGAOs (Candidatus_Competibacter) were responsible for the removal N, and PAOs (Acinetobacter, Candidatus_Accumulibacter, Hypomicrobinm)/DPAOs (Pseudomonas and Dechloromonas) ensured P removal.
同步硝化反硝化与除磷好氧颗粒序批式反应器（SNEDPR-AGSBR）的快速启动和高级脱氮除磷是处理低碳氮（C/N）生活污水的挑战。在这项研究中，在一个非常单级厌氧/好氧/缺氧序批式反应器中考察了 SNEDPR-AGSBR 工艺处理低 C/N 比（3.3-5.0）生活污水的可行性。初步结果表明，伴随着基于对缓慢生长的生物体的选择形成成熟的好氧颗粒污泥，SNEDPR-AGSBR 工艺的快速启动（38 d）成功实现。形成的成熟好氧颗粒具有致密的结构，平均直径为 667.7 μm，SVI 为 30.0 mL/g。长期运行结果揭示了实现聚磷菌/反硝化除磷菌（PAOs/DPAOs）和糖原积累菌/反硝化糖原积累菌（GAOs/DGAOs）之间竞争平衡的两种条件。首先，需要将好氧阶段的溶解氧（DO）浓度降低到 3.0 mg/L，然后，增加好氧和缺氧阶段水力停留时间（HRT）至 3.0 h。值得注意的是，在低 C/N 比为 3.9 的生活污水条件下，SNEDPR-AGSBR 工艺稳定获得了 NH-N（100%）、总氮（84.3%）和 P（91.8%）的高去除效率。在 142 天的长期运行中，实现了 61.6%的同步硝化反硝化（SNED）效率。最后，微生物群落分析证实 GAOs（Defluviicoccus）/DGAOs（Candidatus_Competibacter）负责去除 N，PAOs（不动杆菌、Candidatus_Accumulibacter、Hypomicrobinm）/DPAOs（假单胞菌和脱氯菌）确保 P 的去除。
