Gassman Andrew A, Kao Kenneth K, Bradley James P, Lee Justine C
Division of Plastic and Reconstructive Surgery, Department of Surgery, UCLA David Geffen School of Medicine, CA, USA.
Division of Plastic and Reconstructive Surgery, Department of Surgery, UCLA David Geffen School of Medicine, CA, USA.
J Plast Reconstr Aesthet Surg. 2016 Jul;69(7):959-65. doi: 10.1016/j.bjps.2016.02.007. Epub 2016 Feb 23.
Fat grafting has highly variable long-term results. Research efforts to improve the reliability of fat grafting are limited by inefficient methods for evaluation of fat engraftment. In this work, we describe a novel animal model for the quantitative evaluation of fat grafting using in vivo bioluminescence of adipocytes from luciferase-expressing mice. Subcutaneous adipose tissue from GFP and luciferase-expressing FVB mice were obtained. The samples were homogenized, decanted, and injected into the dorsal skin folds of wild-type FVB mice. Viability of the transferred tissue was examined over a 28-day time period with quantitative bioluminescence after luciferin injection. All animals demonstrated viable adipose transfer with bioluminescence detectable on days 0, 1, 7, 14, and 28. This animal model may be used for noninvasive, longitudinal studies for quantification of the fat engraftment process.
脂肪移植的长期效果差异很大。评估脂肪移植效果的低效方法限制了提高脂肪移植可靠性的研究进展。在这项研究中，我们描述了一种新型动物模型，用于通过表达荧光素酶的小鼠脂肪细胞的体内生物发光对脂肪移植进行定量评估。从表达绿色荧光蛋白（GFP）和荧光素酶的FVB小鼠获取皮下脂肪组织。将样本匀浆、倾析后，注射到野生型FVB小鼠的背部皮褶中。注射荧光素后，在28天的时间内通过定量生物发光检查移植组织的活力。所有动物均显示脂肪移植存活，在第0、1、7、14和28天均可检测到生物发光。该动物模型可用于脂肪移植过程定量的无创纵向研究。
