Jurewicz Joanna, Radwan Michał, Sobala Wojciech, Gromadzińska Jolanta, Jabłońska Ewa, Radwan Paweł, Jakubowski Lucjusz, Wąsowicz Wojciech, Hanke Wojciech
Department of Environmental Epidemiology, Nofer Institute of Occupational Medicine, Lodz, Poland.
Department of Gynecology and Reproduction, "Gameta" Hospital, Rzgów, Poland.
Urology. 2016 Jul;93:86-91. doi: 10.1016/j.urology.2016.03.020. Epub 2016 Mar 24.
To determine whether dietary patterns are associated with the frequency of sperm aneuploidy in a human sperm. It was shown that the role of nutrition, especially dietary pattern, remains unexamined as a risk factor in sperm aneuploidy. In contrast to the traditional analytical approach used in nutritional epidemiology, dietary pattern analysis considers overall diet rather than individual nutrients or foods.
The study population consisted of 212 men who were attending an infertility clinic for diagnostic purposes and who had semen concentration of ≥15 (10(6)/ml) (World Health Organization, 2010). Sperm aneuploidy was assessed using multicolor fluorescent in situ hybridization (DNA probes specific for chromosomes 13, 18, 21, X, Y). Diet was assessed via food frequency questionnaire and dietary patterns were identified by factor analysis. Men were classified into 3 groups according to scores of each dietary pattern: Western, Mixed, Prudent.
In multivariate analysis, Prudent dietary pattern characterized by high intakes of fish, chicken, fruit, cruciferous vegetables, tomatoes, leafy green vegetables, legumes, and whole grains decreases disomy of chromosomes XX and 21 (P = .01 and P = .005) compared with Western dietary pattern characterized by high intakes of red and processed meat, butter, high fat dairy, refined grains, pizza, snacks, high-energy drinks, and sweets.
Higher consumption of Prudent dietary pattern was associated with decreased frequencies of sperm disomy. As this is the first study to analyze the relation of diet and the frequency of sperm aneuploidy, our findings merit further studies, in other populations.
确定饮食模式是否与人类精子非整倍体的频率相关。研究表明，营养尤其是饮食模式作为精子非整倍体的一个风险因素，其作用尚未得到研究。与营养流行病学中使用的传统分析方法不同，饮食模式分析考虑的是整体饮食，而非个别营养素或食物。
研究人群包括212名因诊断目的前往不孕不育诊所就诊且精液浓度≥15（10⁶/ml）的男性（世界卫生组织，2010年）。使用多色荧光原位杂交技术（针对13、18、21、X、Y染色体的特异性DNA探针）评估精子非整倍体情况。通过食物频率问卷评估饮食，并通过因子分析确定饮食模式。根据每种饮食模式的得分将男性分为3组：西方饮食模式组、混合饮食模式组、谨慎饮食模式组。
在多变量分析中，与以高摄入红肉和加工肉类、黄油、高脂肪乳制品、精制谷物、披萨、零食、高能量饮料和甜食为特征的西方饮食模式相比，以高摄入鱼类、鸡肉、水果、十字花科蔬菜、西红柿、绿叶蔬菜、豆类和全谷物为特征的谨慎饮食模式可降低XX和21号染色体的二体性（P = 0.01和P = 0.005）。
较高频率地遵循谨慎饮食模式与精子二体性频率降低有关。由于这是第一项分析饮食与精子非整倍体频率关系的研究，我们的研究结果值得在其他人群中进一步开展研究。
