Koritzinsky Erik H, Street Jonathan M, Star Robert A, Yuen Peter S T
Renal Diagnostics and Therapeutics Unit, NIDDK, NIH, Bethesda, Maryland.
J Cell Physiol. 2017 Jul;232(7):1587-1590. doi: 10.1002/jcp.25387. Epub 2017 Mar 1.
Exosomes are released by cells as self-contained vesicles with an intact lipid bilayer that encapsulates a small portion of the parent cell. Exosomes have been studied widely as information-rich sources of potential biomarkers that can reveal cellular physiology. We suggest that quantification is essential to understand basic biological relationships between exosomes and their parent cells and hence the underlying interpretation of exosome signals. The number of methods for quantifying exosomes has expanded as interest in exosomes has increased. However, a consensus on proper quantification has not developed, making each study difficult to compare to another. Overcoming this ad hoc approach will require widely available standards that have been adequately characterized, and multiple comparative studies across platforms. We outline the current status of these technical approaches and our view of how they can become more coherent. J. Cell. Physiol. 232: 1587-1590, 2017. © 2016 Wiley Periodicals, Inc.
外泌体由细胞释放，是一种具有完整脂质双层的独立囊泡，包裹着一小部分亲代细胞。外泌体作为富含信息的潜在生物标志物来源已被广泛研究，这些标志物能够揭示细胞生理学特性。我们认为，定量分析对于理解外泌体与其亲代细胞之间的基本生物学关系至关重要，从而对外泌体信号进行潜在解读。随着对外泌体兴趣的增加，用于定量外泌体的方法数量也在增加。然而，尚未就适当的定量方法达成共识，这使得每项研究都难以相互比较。克服这种临时方法需要广泛可用且已充分表征的标准，以及跨平台的多项比较研究。我们概述了这些技术方法的现状以及我们对如何使其更加连贯一致的看法。《细胞生理学杂志》232: 1587 - 1590, 2017年。© 2016威利期刊公司。
