Noboa-Velástegui Jacqueline, León Juan Carlos, Castro Jorge, Fletes Ana, Madrigal Perla, Álvarez Iñaki, Navarro Rosa
Doctorado en Ciencias Biomédicas, Secretaría Académica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Calle Sierra Mojada No. 950, Colonia Independencia, Guadalajara C.P. 44340, Mexico.
Departamento de Biología Celular, Fisiología e Inmnunología, Institut de Biotecnologia i Biomedicina, Campus de Bellaterra, Bellatera, 08193 Barcelona, Spain.
Life (Basel). 2025 Mar 6;15(3):410. doi: 10.3390/life15030410.
Adipose tissue is responsible for fat storage and is an important producer of extracellular vesicles (EVs). The biological content of exosomes, one kind of EV, provides information on aspects such as immunometabolic alterations. This study aimed to compare three plasma exosome isolation methods-using a commercial kit (CK), size exclusion chromatography (SEC), and differential centrifugation (DC)-and select the best one. Individuals categorized by normal and high body fat percentages were used. The DC and CK were proven to be the most advantageous out of the exosome isolation methods, so we suggest these methods for further protein and molecular analyses, respectively. Still, we emphasize the importance of selecting an appropriate methodology depending on the specific research objectives. At the same time, no statistical differences in exosome quality, morphology, total protein, or concentration were observed between individuals categorized by body fat percentage, so we suggest that the exosomal cargo varies in individuals with normal and high fat percentages.
脂肪组织负责脂肪储存，是细胞外囊泡（EVs）的重要产生者。外泌体是EVs的一种，其生物学内容提供了有关免疫代谢改变等方面的信息。本研究旨在比较三种血浆外泌体分离方法——使用商业试剂盒（CK）、尺寸排阻色谱法（SEC）和差速离心法（DC）——并选择最佳方法。使用了按正常和高体脂百分比分类的个体。在所有外泌体分离方法中，DC和CK被证明是最具优势的，因此我们分别建议将这些方法用于进一步的蛋白质和分子分析。不过，我们强调根据具体研究目标选择合适方法的重要性。同时，按体脂百分比分类的个体之间在外泌体质量、形态、总蛋白或浓度方面未观察到统计学差异，因此我们认为正常和高脂肪百分比个体的外泌体货物存在差异。
