Manoharan Aarthi, Rajkumar Ravi Philip, Shewade Deepak Gopal, Sundaram Rajan, Muthuramalingam Avin, Paul Abialbon
Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
Department of Psychiatry, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
Hum Psychopharmacol. 2016 May;31(3):178-84. doi: 10.1002/hup.2525. Epub 2016 Mar 27.
Only 30% of major depressive disorder (MDD) patients achieve complete remission with a serotonergic antidepressant (selective serotonin reuptake inhibitor). We investigated the potential of serotonin (5-HT) and interleukin-6 (IL-6) to serve as functional biomarkers of fluoxetine response.
Serum IL-6 and 5-HT were measured in 73 MDD patients (39 responders and 34 non-responders) pre- and 6 weeks post-treatment and in 44 normal controls with ELISA. Fluoxetine and norfluoxetine were measured using LC MS/MS.
IL-6 levels were significantly higher in MDD patients when compared with controls (p < 0.01), and 5-HT levels were significantly lower in non-responders compared with controls (p = 0.0131). Pre- and post-treatment levels of both biomarkers individually and in combination did not significantly differ between responders and non-responders. Area under the receiver operating characteristics curve for the biomarkers was 0.5. Significant correlation was seen between the percentage change in IL-6 and percentage change in Hamilton Rating Scale for Depression score in responders. Fluoxetine and norfluoxetine concentrations were not significantly different in responders and non-responders, and there was no correlation between fluoxetine concentrations and percentage reduction in 5-HT from week 0 to 6.
5-HT and IL-6 may not serve as useful markers of response to fluoxetine because of inconsistent results across different studies. Copyright © 2016 John Wiley & Sons, Ltd.
在使用血清素能抗抑郁药（选择性5-羟色胺再摄取抑制剂）治疗的重度抑郁症（MDD）患者中，仅有30%能实现完全缓解。我们研究了血清素（5-HT）和白细胞介素-6（IL-6）作为氟西汀反应功能生物标志物的潜力。
采用酶联免疫吸附测定法（ELISA），对73例MDD患者（39例反应者和34例无反应者）治疗前及治疗6周后的血清IL-6和5-HT水平进行了检测，并与44名正常对照者进行了比较。使用液相色谱-串联质谱法（LC MS/MS）测定氟西汀和去甲氟西汀。
与对照组相比，MDD患者的IL-6水平显著更高（p < 0.01），与对照组相比，无反应者的5-HT水平显著更低（p = 0.0131）。反应者和无反应者之间，这两种生物标志物单独及联合检测的治疗前和治疗后水平均无显著差异。生物标志物的受试者工作特征曲线下面积为0.5。反应者中，IL-6变化百分比与汉密尔顿抑郁量表评分变化百分比之间存在显著相关性。反应者和无反应者的氟西汀和去甲氟西汀浓度无显著差异，且从第0周到第6周，氟西汀浓度与5-HT降低百分比之间无相关性。
由于不同研究结果不一致，5-HT和IL-6可能无法作为氟西汀反应的有效标志物。版权所有© 2016约翰威立父子有限公司。
