Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.
Faculty of Medical Science, Department of Microbiology and Parasitology, Naresuan University, Phitsanulok, Thailand.
J Med Virol. 2016 Oct;88(10):1785-90. doi: 10.1002/jmv.24536. Epub 2016 Apr 5.
The hepatitis C virus (HCV) is an important cause of liver dysfunction which continues to spread in Thailand, particularly as genotype 6. The NS5B gene fragment is particularly variable and thus provides a valuable tracker for its spread. Therefore, the purpose of this study was to characterize the HCV genotype 6 based on partial NS5B region using Thai blood donor samples. Twenty-nine samples were genotyped as HCV 6 by nested PCR, nucleotide sequencing and amino acid sequence analysis. Amplified products were identified as HCV genotypes 6f, 6c, 6n, and 6i. There were amino acid variations of 4-18 residues in subtypes 6f, 6c, and 6n whereas subtype 6i was conserved when compared with their referent strains. In subtypes 6f, 6c, 6n, and 6i, the amino acid mutations at positions 244, 309, and 310 which are associated with HCV resistance were present. In summary, the sequences and phylogenetic analysis of NS5B of HCV used in our study yielded the genotypes 6f, 6c, 6n, and 6i. This finding indicates diversity of amino acids in NS5B of HCV. Characterizing the partial NS5B region among hepatitis C virus genotype 6 subtypes may predict efficacious anti-HCV therapy. J. Med. Virol. 88:1785-1790, 2016. © 2016 Wiley Periodicals, Inc.
丙型肝炎病毒（HCV）是导致肝功能障碍的重要原因，在泰国仍在继续传播，尤其是基因型 6。NS5B 基因片段特别多变，因此为其传播提供了有价值的追踪器。因此，本研究的目的是使用泰国献血者样本，根据部分 NS5B 区域对 HCV 基因型 6 进行特征描述。通过巢式 PCR、核苷酸测序和氨基酸序列分析，将 29 个样本鉴定为 HCV 6 型。扩增产物被鉴定为 HCV 6f、6c、6n 和 6i 型。在 6f、6c 和 6n 亚型中，有 4-18 个残基的氨基酸变异，而与参照株相比，6i 亚型保守。在 6f、6c、6n 和 6i 亚型中，存在与 HCV 耐药性相关的位置 244、309 和 310 的氨基酸突变。总之，我们研究中使用的 HCV NS5B 的序列和系统发生分析产生了 6f、6c、6n 和 6i 基因型。这一发现表明 HCV NS5B 中的氨基酸多样性。对丙型肝炎病毒基因型 6 亚型的 NS5B 部分进行特征描述可能有助于预测有效的抗 HCV 治疗效果。J. Med. Virol. 88:1785-1790, 2016. © 2016 Wiley Periodicals, Inc.
