Lu Ling, Li Chunhua, Fu Yongshui, Thaikruea Lakkana, Thongswat Satawat, Maneekarn Niwat, Apichartpiyakul Chatchawann, Hotta Hak, Okamoto Hiroaki, Netski Dale, Pybus Oliver G, Murphy Donald, Hagedorn Curt H, Nelson Kenrad E
Division of Gastroenterology/Hepatology, Department of Medicine, University of Kansas Medical Center, 4035 Delp, MS 1023, Kansas City, KS 66160, USA.
The First People's Hospital of Yunnan Province, Kunming, China.
J Gen Virol. 2007 May;88(Pt 5):1505-1518. doi: 10.1099/vir.0.82604-0.
In this study, the first complete genome sequences for hepatitis C virus (HCV) subtypes 6f, 6i, 6j and 6m, obtained from infected blood donors in Chiang Mai, Thailand, are reported. Pairwise genome-wide nucleotide similarities between some of these isolates were higher than the 75-80% value used previously to define different HCV subtypes. To investigate further, the entire genomes of four prototype isolates, Th602 (6i), Th553 (6j), B4/92 (6m) and D86/93 (6n), were sequenced. Pairwise comparison of these sequences gave a similar range of nucleotide similarities, thereby providing new information for HCV subtype classification. In order to study the hypothesis of interspousal HCV transmission, four additional complete HCV genome sequences were obtained from two infected Thai blood donors and their spouses, C-0044 and C-0046 (6f), and C-0192 and C-0185 (6m). Pairwise comparison of the sequences revealed that C-0044 and C-0046 share a nucleotide similarity of 98.1%, whilst C-0185 and C-0192 have a similarity of 97.8%. Several other studies of partial HCV sequences of different genomic regions from HCV-infected couples have shown nucleotide similarities ranging from 96.3 to 100%. The similarities of the complete genome sequences from the two couples in the current study are consistent with HCV transmission between spouses.
在本研究中，报告了从泰国清迈感染献血者中获得的丙型肝炎病毒（HCV）6f、6i、6j和6m亚型的首个完整基因组序列。这些分离株中某些之间的全基因组核苷酸两两相似性高于先前用于定义不同HCV亚型的75 - 80%的值。为进一步研究，对四个原型分离株Th602（6i）、Th553（6j）、B4/92（6m）和D86/93（6n）的全基因组进行了测序。这些序列的两两比较给出了相似的核苷酸相似性范围，从而为HCV亚型分类提供了新信息。为了研究配偶间HCV传播的假说，从两名受感染的泰国献血者及其配偶C - 0044和C - 0046（6f）以及C - 0192和C - 0185（6m）中又获得了四个完整的HCV基因组序列。序列的两两比较显示，C - 0044和C - 0046的核苷酸相似性为98.1%，而C - 0185和C - 0192的相似性为97.8%。其他几项对HCV感染夫妇不同基因组区域的HCV部分序列的研究显示核苷酸相似性范围为96.3%至100%。本研究中两对夫妇的完整基因组序列相似性与配偶间的HCV传播一致。
