Li Xin, Dai Yingnan, Yan Shujun, Shi Yanli, Li Jingxiu, Liu Jinglu, Cha Li, Mu Jianjun
a Department of Cardiology , The First Affiliated Hospital of Medical College, Xi'an Jiao Tong University , Xi'an , China.
b Department of Cardiology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China.
Clin Exp Hypertens. 2016;38(3):287-93. doi: 10.3109/10641963.2015.1089882. Epub 2016 Mar 28.
Resveratrol, a polyphenol of natural compounds, has beneficial cardiovascular effects, many of which are mediated by nitric oxide (NO). Resveratrol increases intracellular calcium and activates AMP-activated protein kinase (AMPK), all of which could increase NO production. We hypothesized that resveratrol via a calcium-dependent NO production lowers blood pressure (BP) in spontaneously hypertensive rats (SHR).
Acetylcholine (Ach)-induced endothelium-dependent relaxations in rat aortas were examined by organ chamber. Blood pressures were determined by radiotelemetry methods.
Incubation of isolated aortas from SHR with resveratrol dramatically improved vasorelaxation induced by Ach. Preincubation of aortas with endothelial NO synthase (eNOS) inhibitor or calcium chelant blunted the effects of resveratrol on Ach-induced relaxation, as wells as NO production and eNOS phosphorylation. In animal studies, administration of resveratrol significantly lowered systemic BP in SHR.
Resveratrol increases endothelial NO production to improve endothelial dysfunction and lowers BP in hypertensive rats, which depends on calcium-eNOS activation.
白藜芦醇是一种天然化合物多酚，具有有益的心血管效应，其中许多是由一氧化氮（NO）介导的。白藜芦醇可增加细胞内钙并激活AMP激活的蛋白激酶（AMPK），所有这些都可能增加NO的产生。我们假设白藜芦醇通过钙依赖性NO产生降低自发性高血压大鼠（SHR）的血压（BP）。
通过器官浴槽检测乙酰胆碱（Ach）诱导的大鼠主动脉内皮依赖性舒张。通过无线电遥测法测定血压。
用白藜芦醇孵育SHR的离体主动脉可显著改善Ach诱导的血管舒张。用内皮型NO合酶（eNOS）抑制剂或钙螯合剂预孵育主动脉可减弱白藜芦醇对Ach诱导舒张的作用，以及NO产生和eNOS磷酸化。在动物研究中，给予白藜芦醇可显著降低SHR的全身血压。
白藜芦醇增加内皮NO产生以改善内皮功能障碍并降低高血压大鼠的血压，这依赖于钙-eNOS激活。
