Ren Dahai, Xia Yiqiu, Wang Bin, You Zheng
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University , Beijing, 100084, China.
Anal Chem. 2016 Apr 19;88(8):4318-27. doi: 10.1021/acs.analchem.5b04471. Epub 2016 Apr 7.
Quantum dot (QD) multicolor analysis was achieved by labeling the epidermal growth factor and labeling the antibody against the epidermal growth factor receptor (anti-EGFR) using different QDs. On the basis of the fluorescence intensity of the two types of QDs, the amount of epidermal growth factors (EGFs) in the cells and anti-EGFRs bound to EGFRs were analyzed. The functions of anti-EGFR in preventing HeLa cells from engulfing EGF and inhibiting overproliferation of the HeLa cells were also studied. Meanwhile, parallel analysis was conducted to analyze the heterogeneity of cells at the single-cell level using a single-cell array. This provides a novel approach for the multiplexed analysis for the anti-EGFR functions in cells together with the cell heterogeneity. It also lays a foundation for parallel analysis and detection, using various fluorescence probes, simultaneous tracking, and detection of multiple targets at an overall level or individual level, and multichannel analysis of drug effects.
通过使用不同的量子点标记表皮生长因子和抗表皮生长因子受体抗体（抗EGFR），实现了量子点（QD）多色分析。基于两种量子点的荧光强度，分析了细胞中表皮生长因子（EGF）的量以及与EGFR结合的抗EGFR的量。还研究了抗EGFR在阻止HeLa细胞吞噬EGF和抑制HeLa细胞过度增殖方面的功能。同时，使用单细胞阵列进行平行分析，以在单细胞水平分析细胞的异质性。这为细胞中抗EGFR功能与细胞异质性的多重分析提供了一种新方法。它还为使用各种荧光探针进行平行分析和检测、在整体水平或个体水平同时跟踪和检测多个靶标以及药物作用的多通道分析奠定了基础。
