Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China.
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China; Institute of Biotranslational Medicine, Jinan University, Guangzhou 510632, PR China.
Biosens Bioelectron. 2016 Jul 15;81:524-531. doi: 10.1016/j.bios.2016.03.049. Epub 2016 Mar 20.
Microplate readers are important diagnostic instruments, used intensively for various readout test kits (biochemical analysis kits and ELISA kits). However, due to their expensive and non-portability, commercial microplate readers are unavailable for home testing, community and rural hospitals, especially in developing countries. In this study, to provide a field-portable, cost-effective and versatile diagnostic tool, we reported a novel smart phone based microplate reader. The basic principle of this devise relies on a smart phone's optical sensor that measures transmitted light intensities of liquid samples. To prove the validity of these devises, developed smart phone based microplate readers were applied to readout results of various analytical targets. These targets included analanine aminotransferase (ALT; limit of detection (LOD) was 17.54 U/L), alkaline phosphatase (AKP; LOD was 15.56 U/L), creatinine (LOD was 1.35μM), bovine serum albumin (BSA; LOD was 0.0041mg/mL), prostate specific antigen (PSA; LOD was 0.76pg/mL), and ractopamine (Rac; LOD was 0.31ng/mL). The developed smart phone based microplate readers are versatile, portable, and inexpensive; they are unique because of their ability to perform under circumstances where resources and expertize are limited.
微孔板读数仪是一种重要的诊断仪器，广泛应用于各种读取测试试剂盒（生化分析试剂盒和 ELISA 试剂盒）。然而，由于其昂贵且不便于携带，商业微孔板读数仪无法用于家庭检测、社区和农村医院，特别是在发展中国家。在这项研究中，为了提供一种现场便携、经济高效且多功能的诊断工具，我们报告了一种基于智能手机的新型微孔板读数仪。该设备的基本原理依赖于智能手机的光学传感器，该传感器可测量液体样本的透射光强度。为了验证这些设备的有效性，我们开发的基于智能手机的微孔板读数仪被用于读取各种分析物的结果。这些分析物包括丙氨酸氨基转移酶（ALT；检测限（LOD）为 17.54 U/L）、碱性磷酸酶（AKP；LOD 为 15.56 U/L）、肌酸（LOD 为 1.35μM）、牛血清白蛋白（BSA；LOD 为 0.0041mg/mL）、前列腺特异性抗原（PSA；LOD 为 0.76pg/mL）和莱克多巴胺（Rac；LOD 为 0.31ng/mL）。开发的基于智能手机的微孔板读数仪用途广泛、便携且价格低廉；其独特之处在于，它们能够在资源和专业知识有限的情况下进行检测。
