State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, 510006, China.
The Second Affiliated Hospital Zhejiang University School of Medicine, Department of Clinical Medical Engineering, Hangzhou, 310009, China.
Talanta. 2023 Jan 15;252:123862. doi: 10.1016/j.talanta.2022.123862. Epub 2022 Aug 24.
Facile, efficient, and inexpensive biosensing systems are in high demand for biomedical test. In recent years, numerous smartphone-based biosensing systems have been developed to match demand for biomedical test in source-limited environment. However, application of these smartphone-based biosensing systems was limited because of performance gap between the smartphone-based systems and commercial plate readers. In this study, we have developed a smart tablet-phone-based colorimetric plate reader (STPCPR) with intelligent and dynamic light modulation for broad-range colorimetric assays. The STPCPR allows controllable modulation of exciting light in three different color channels that is lack in conventional smartphone-based system. Using optimized exciting modulation, the STPCPR shows higher sensitivities, lower detection limits, and broader detection ranges in test of pigments, proteins, and cells when compared to conventional plate readers and smartphone-based system. Therefore, the developed STPCPR can serve as an ideal next-generation smartphone-based biosensing system for point-of-care colorimetric test in diverse biomedical applications in source-limited environment.
用于生物医学检测的简便、高效、廉价的生物传感系统需求量很大。近年来，为了满足资源有限环境下对生物医学检测的需求，已经开发出了许多基于智能手机的生物传感系统。然而，由于基于智能手机的系统与商用板读数器之间存在性能差距，这些基于智能手机的生物传感系统的应用受到了限制。在本研究中，我们开发了一种具有智能和动态光调制功能的智能平板手机比色板读数器（STPCPR），可用于广泛的比色分析。STPCPR 允许在三个不同的颜色通道中控制激发光的调制，而传统的基于智能手机的系统则缺乏这种功能。与传统的板读数器和基于智能手机的系统相比，使用优化的激发调制，STPCPR 在测试颜料、蛋白质和细胞时具有更高的灵敏度、更低的检测限和更宽的检测范围。因此，所开发的 STPCPR 可以作为一种理想的下一代基于智能手机的生物传感系统，用于资源有限环境下的各种生物医学应用中的即时比色检测。
