Borzoui E, Naseri B
Department of Plant Protection,Faculty of Agricultural Sciences,University of Mohaghegh Ardabili,Ardabil,Iran.
Bull Entomol Res. 2016 Aug;106(4):464-73. doi: 10.1017/S000748531600016X. Epub 2016 Mar 28.
The life history and digestive α-amylase activity of the Angoumois grain moth, Sitotroga cerealella Olivier (Lepidoptera: Gelechiidae) were studied on six wheat cultivars (Arg, Bam, Nai 60, Pishtaz, Sepahan and Shanghai) at 25 ± 1°C, relative humidity of 65 ± 5% and a photoperiod of 16:8 (L:D) h. A delay in the developmental time of S. cerealella immature stages was detected when larvae were fed on cultivar Sepahan. The maximum survival rate of immature stages was seen on cultivar Bam (93.33 ± 2.10%), and the minimum rates were on cultivars Nai 60 (54.66 ± 2.49%) and Sepahan (49.33 ± 4.52%). The highest realized fecundity and fertility were recorded for females which came from larvae fed on cultivar Bam (93.30 ± 2.10 eggs/female and 91.90 ± 3.10%, respectively); and the lowest ones were observed for females which came from larvae fed on cultivar Sepahan (49.30 ± 4.50 eggs/female and 67.4 ± 11.1%, respectively). The heaviest male and female weights of S. cerealella were observed on cultivar Bam (2.97 ± 0.02 and 4.80 ± 0.01 mg, respectively). The highest amylolytic activity of the fourth instar was detected on cultivar Bam (0.89 ± 0.04 mg maltose min-1), which had the maximum mean hundred-wheat weight (5.92 ± 0.19 g). One α-amylase isozyme was detected in the midgut extracts from the fourth instar larvae fed on different wheat cultivars, and the highest intensity was found in larvae fed on cultivar Bam. Correlation analyses showed that very high correlations existed between the immature period, fecundity and fertility on one side and inhibition of α-amylase, soluble starch content and hundred-wheat weight on the other. According to the obtained results, cultivar Sepahan is an unfavorable host for the feeding and development of S. cerealella.
在温度25±1°C、相对湿度65±5%、光周期16:8（光照:黑暗）小时的条件下，研究了麦蛾（Sitotroga cerealella Olivier，鳞翅目：麦蛾科）在六个小麦品种（阿尔格、巴姆、奈60、皮什塔兹、赛帕汉和上海）上的生活史及消化α-淀粉酶活性。当幼虫取食赛帕汉品种时，麦蛾未成熟阶段的发育时间出现延迟。未成熟阶段最高存活率出现在巴姆品种上（93.33±2.10%），最低存活率出现在奈60品种（54.66±2.49%）和赛帕汉品种（49.33±4.52%）上。来自取食巴姆品种幼虫的雌蛾实现了最高的繁殖力和生育力（分别为93.30±2.10粒卵/雌蛾和91.90±3.10%）；来自取食赛帕汉品种幼虫的雌蛾的繁殖力和生育力最低（分别为49.30±4.50粒卵/雌蛾和67.4±11.1%）。麦蛾最重的雄蛾和雌蛾体重出现在巴姆品种上（分别为2.97±0.02毫克和4.80±0.01毫克）。四龄幼虫的最高淀粉酶活性出现在巴姆品种上（0.89±0.04毫克麦芽糖分钟-1），该品种的平均百粒小麦重量最高（5.92±0.19克）。在取食不同小麦品种的四龄幼虫中肠提取物中检测到一种α-淀粉酶同工酶，在取食巴姆品种的幼虫中发现其强度最高。相关性分析表明，一方面未成熟期、繁殖力和生育力与另一方面α-淀粉酶抑制、可溶性淀粉含量和百粒小麦重量之间存在非常高的相关性。根据所得结果，赛帕汉品种是麦蛾取食和发育的不利寄主。
