Golikhajeh Neshat, Naseri Bahram, Razmjou Jabraeil
Department of Plant Protection, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
Department of Plant Protection, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
J Insect Sci. 2017 Jan 27;17(1). doi: 10.1093/jisesa/iew104. Print 2017 Jan.
Digestive enzymatic activity in three geographic strains (Miandiab, Kalposh and Moghan regions) of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) reared on different sugar beet cultivars (Dorothea, Rozier, Persia and Perimer) was studied under laboratory conditions (25 ± 1 °C, 65 ± 5% RH, and a photo period of 16:8 (L:D) h photoperiod). The results of this study demonstrated that digestive protease and amylase activity of S. exigua larvae was affected by both geographic origin of the pest and host plant cultivar. Three strains reared on the same sugar beet cultivars demonstrated different levels of proteolytic and amylolytic activities in fourth and fifth instars. The highest proteolytic and amylolytic activity, in most cases, was observed in larvae collected from Kalposh region. Among different sugar beet cultivars, the highest protease activity in three strains was observed on cultivars Rozier and Perimer. Nevertheless, the highest amylase activity was seen on cultivar Dorothea, and the lowest activity was seen on cultivar Rozier. This study suggested that variations in digestive enzymatic activity of three geographic strains of S. exigua might be attributed to local adaptation with their local host plant and environmental conditions inherent by larvae.
在实验室条件（25±1°C、65±5%相对湿度、16:8（光照:黑暗）小时光周期）下，研究了在不同甜菜品种（多萝西娅、罗齐尔、波斯和佩里默）上饲养的甜菜夜蛾（鳞翅目：夜蛾科）三个地理品系（米安迪亚布、卡尔波什和莫加恩地区）的消化酶活性。本研究结果表明，甜菜夜蛾幼虫的消化蛋白酶和淀粉酶活性受害虫地理起源和寄主植物品种的影响。在相同甜菜品种上饲养的三个品系在四龄和五龄时表现出不同水平的蛋白水解和淀粉水解活性。在大多数情况下，从卡尔波什地区采集的幼虫中观察到最高的蛋白水解和淀粉水解活性。在不同的甜菜品种中，三个品系在罗齐尔和佩里默品种上观察到最高的蛋白酶活性。然而，在多萝西娅品种上观察到最高的淀粉酶活性，在罗齐尔品种上观察到最低的活性。本研究表明，甜菜夜蛾三个地理品系消化酶活性的差异可能归因于幼虫对当地寄主植物的局部适应及其所处的环境条件。
