Ko Chun Hay, Lau Kit Man, Chau Lily, Cheung David Wing Shing, Siu Wing Sum, Wong Hing Lok, Shum Wai Ting, Gao Si, Fung Kwok Pui, Leung Ping Chung, Poon Simon Kar-Sing, Lau Clara Bik San
Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
J Pharm Pharmacol. 2016 Jun;68(6):826-33. doi: 10.1111/jphp.12542. Epub 2016 Mar 28.
An anti-osteoporotic herbal formula ELP containing Epimedii Herba (E), Ligustri Lucidi Fructus (L) and Psoraleae Fructus (P) was studied to investigate the herb-herb interaction (or the possible synergistic effect) among each component and to identify the principal herbs in different modes of action.
Rat osteoblast-like UMR-106 cells proliferation, rat MSCs-derived osteoblastogenesis and RANKL-induced RAW 264.7 osteoclastogenesis were adopted to investigate the bone-forming activity and bone-degrading activity of the herbal extracts. In the statistical aspect, a modified Tallarida's approach was employed to assess the synergistic effects in herbal combinations.
Psoraleae Fructus is the active herb for stimulating osteoblast proliferation, and mild synergy was detected in the pairwise combinations EL, LP and formula ELP. In osteoblastogenesis assay, E and L are the principal herbs for promoting osteoblast differentiation and significant synergy was detected in the pairwise combination EL. For inhibiting osteoclast formation, L is the active herb and significant synergy was detected in the 3-way combination ELP.
The presence of E, L and P is essential for ELP formula as a whole to act against osteoporosis via enhancing bone formation and reducing bone reabsorption. An optimal dosage at 150 μg/ml was proposed for ELP based on our findings.
研究一种含淫羊藿（E）、女贞子（L）和补骨脂（P）的抗骨质疏松草药配方ELP，以探讨各成分之间的草药-草药相互作用（或可能的协同作用），并确定不同作用模式下的主要草药。
采用大鼠成骨样UMR-106细胞增殖、大鼠间充质干细胞衍生的成骨细胞生成和RANKL诱导的RAW 264.7破骨细胞生成，来研究草药提取物的成骨活性和骨降解活性。在统计学方面，采用改良的塔拉瑞达方法评估草药组合中的协同作用。
补骨脂是刺激成骨细胞增殖的活性草药，在EL、LP和配方ELP的两两组合中检测到轻微协同作用。在成骨细胞生成试验中，E和L是促进成骨细胞分化的主要草药，在EL的两两组合中检测到显著协同作用。对于抑制破骨细胞形成，L是活性草药，在ELP三联组合中检测到显著协同作用。
E、L和P的存在对于ELP配方整体通过增强骨形成和减少骨吸收来对抗骨质疏松至关重要。基于我们的研究结果，建议ELP的最佳剂量为150μg/ml。
