Choi Se Hoon, Kim Young Hye, D'Avanzo Carla, Aronson Jenna, Tanzi Rudolph E, Kim Doo Yeon
Genetics and Aging Research Unit, MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
Biomedical Omics Group, Korea Basic Science Institute, Cheongju-si, Chungbuk 363-883, Republic of Korea.
US Neurol. 2015 Fall;11(2):102-105. doi: 10.17925/USN.2015.11.02.102.
The "amyloid β hypothesis" of Alzheimer's disease (AD) has been the reigning hypothesis explaining pathogenic mechanisms of AD over the last two decades. However, this hypothesis has not been fully validated in animal models, and several major unresolved issues remain. We recently developed a human neural cell culture model of AD based on a three-dimensional (3D) cell culture system. This unique, cellular model recapitulates key events of the AD pathogenic cascade, including β-amyloid plaques and neurofibrillary tangles. Our 3D human neural cell culture model system provides a premise for a new generation of cellular AD models that can serve as a novel platform for studying pathogenic mechanisms and for high-throughput drug screening in a human brain-like environment.
在过去二十年里，阿尔茨海默病（AD）的“淀粉样蛋白β假说”一直是解释AD致病机制的主导假说。然而，这一假说在动物模型中尚未得到充分验证，仍存在几个主要的未解决问题。我们最近基于三维（3D）细胞培养系统开发了一种AD的人类神经细胞培养模型。这个独特的细胞模型概括了AD致病级联反应的关键事件，包括β淀粉样斑块和神经原纤维缠结。我们的3D人类神经细胞培养模型系统为新一代细胞AD模型提供了前提条件，这些模型可作为研究致病机制以及在类似人脑环境中进行高通量药物筛选的新型平台。
