Ghani Wan Mohd Hafezul Wan Abdul, Rawi Che Salmah Md, Hamid Suhaila Abd, Al-Shami Salman Abdo
Trop Life Sci Res. 2016 Feb;27(1):115-33.
This study analyses the sampling performance of three benthic sampling tools commonly used to collect freshwater macroinvertebrates. Efficiency of qualitative D-frame and square aquatic nets were compared to a quantitative Surber sampler in tropical Malaysian streams. The abundance and diversity of macroinvertebrates collected using each tool evaluated along with their relative variations (RVs). Each tool was used to sample macroinvertebrates from three streams draining different areas: a vegetable farm, a tea plantation and a forest reserve. High macroinvertebrate diversities were recorded using the square net and Surber sampler at the forested stream site; however, very low species abundance was recorded by the Surber sampler. Relatively large variations in the Surber sampler collections (RVs of 36% and 28%) were observed for the vegetable farm and tea plantation streams, respectively. Of the three sampling methods, the square net was the most efficient, collecting a greater diversity of macroinvertebrate taxa and a greater number of specimens (i.e., abundance) overall, particularly from the vegetable farm and the tea plantation streams (RV<25%). Fewer square net sample passes (<8 samples) were sufficient to perform a biological assessment of water quality, but each sample required a slightly longer processing time (±20 min) compared with those gathered via the other samplers. In conclusion, all three apparatuses were suitable for macroinvertebrate collection in Malaysian streams and gathered assemblages that resulted in the determination of similar biological water quality classes using the Family Biotic Index (FBI) and the Biological Monitoring Working Party (BMWP). However, despite a slightly longer processing time, the square net was more efficient (lowest RV) at collecting samples and more suitable for the collection of macroinvertebrates from deep, fast flowing, wadeable streams with coarse substrates.
本研究分析了三种常用于采集淡水大型无脊椎动物的底栖采样工具的采样性能。在马来西亚热带溪流中，将定性的D形网和方形水生网的效率与定量的 Surber采样器进行了比较。对使用每种工具采集的大型无脊椎动物的丰度和多样性及其相对变化（RV）进行了评估。每种工具都用于从三条流经不同区域的溪流中采样大型无脊椎动物：一个蔬菜农场、一个茶园和一个森林保护区。在森林溪流站点，使用方形网和Surber采样器记录到了较高的大型无脊椎动物多样性；然而，Surber采样器记录到的物种丰度非常低。分别在蔬菜农场溪流和茶园溪流中观察到Surber采样器采集结果的相对较大变化（RV分别为36%和
28%）。在这三种采样方法中，方形网效率最高，总体上采集到了更多种类的大型无脊椎动物分类群和更多数量的标本（即丰度），特别是从蔬菜农场和茶园溪流中采集的标本（RV<25%）。较少的方形网采样次数（<8个样本）就足以进行水质的生物学评估，但与通过其他采样器采集的样本相比，每个样本的处理时间略长（±20分钟）。总之，所有三种仪器都适用于在马来西亚溪流中采集大型无脊椎动物，并且采集到的组合通过家庭生物指数（FBI）和生物监测工作组（BMWP）得出了相似的生物水质类别。然而，尽管处理时间略长，但方形网在采集样本方面更高效（RV最低），更适合从具有粗糙基质的深、水流湍急且可涉水的溪流中采集大型无脊椎动物。
