Department of Electrical Engineering, National Chung Hsing University, Taichung 40227, Taiwan.
Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan.
Biosens Bioelectron. 2017 Mar 15;89(Pt 1):511-517. doi: 10.1016/j.bios.2016.03.046. Epub 2016 Mar 19.
In this work, we developed a field effect transistor (FET) biosensor utilizing solution-processed graphene oxide nanoribbon (GONR) for methylene blue (MB) sensing. MB is a unique material; one of its crucial applications is as a marker in the detection of biomaterials. Therefore, a highly sensitive biosensor with a low detection limit that can be fabricated simply in a noncomplex detection scheme is desirable. GONR is made by unzipping multiwall carbon nanotubes, which can be mass-produced at low temperature. The GONR-FET biosensor demonstrated a sensitivity of 12.5μA/mM (determined according to the drain current difference caused by the MB concentration change). The Raman spectra indicate that the materials quality of the GONR and the domain size for the C=C sp bonding were both improved after MB detection. X-ray photoelectron spectroscopy revealed that the hydroxyl groups on the GONR were removed by the reductive MB. According to XPS and Raman, the positive charge is proposed to transfer from MB to GONR during sensing. This transfer causes charge in-neutrality in the GONR which is compensated by releasing •OH functional groups. With high sensitivity, a low detection limit, and a simple device structure, the GONR-FET sensor is suitable for sensing biomaterials.
在这项工作中，我们开发了一种场效应晶体管（FET）生物传感器，利用溶液处理的氧化石墨烯纳米带（GONR）用于亚甲基蓝（MB）传感。MB 是一种独特的材料；其重要应用之一是作为生物材料检测中的标记物。因此，需要一种能够在非复杂检测方案中简单制造、具有低检测限和高灵敏度的生物传感器。GONR 是通过解开多壁碳纳米管制成的，可以在低温下大规模生产。GONR-FET 生物传感器的灵敏度为 12.5μA/mM（根据由 MB 浓度变化引起的漏电流差异确定）。拉曼光谱表明，MB 检测后，GONR 的材料质量和 C=C sp 键的畴尺寸都得到了改善。X 射线光电子能谱表明，GONR 上的羟基基团被还原的 MB 去除。根据 XPS 和拉曼，在传感过程中，正电荷被提议从 MB 转移到 GONR。这种转移导致 GONR 中的电荷中性，通过释放•OH 官能团来补偿。由于具有高灵敏度、低检测限和简单的器件结构，GONR-FET 传感器适合用于生物材料传感。
