Martins Carlos Henrique Gomes, Pires Regina Helena, Cunha Aline Oliveira, Pereira Cristiane Aparecida Martins, Singulani Junya de Lacorte, Abrão Fariza, Moraes Thais de, Mendes-Giannini Maria José Soares
Laboratorio de Pesquisa em Microbiologia Aplicada, Universidade de Franca, UNIRAN, Av. Dr. Armando Salles de Oliveira, 201, Pq. Universitário, Franca, SP 14404-600, Brazil.
Laboratorio de Pesquisa em Microbiologia Aplicada, Universidade de Franca, UNIRAN, Av. Dr. Armando Salles de Oliveira, 201, Pq. Universitário, Franca, SP 14404-600, Brazil; Universidade Estadual Paulista "Júlio de Mesquita Filho", Faculdade de Ciências Farmacêuticas, UNESP, Rodovia Ararquara-Jaú, km 1, Araraquara, SP 14.902-801, Brazil.
Fungal Biol. 2016 Apr;120(4):530-537. doi: 10.1016/j.funbio.2016.01.013. Epub 2016 Feb 4.
Denture liners have physical properties that favour plaque accumulation and colonization by Candida species, irritating oral tissues and causing denture stomatitis. To isolate and determine the incidence of oral Candida species in dental prostheses, oral swabs were collected from the dental prostheses of 66 patients. All the strains were screened for their ability to form biofilms; both monospecies and dual-species combinations were tested. Candida albicans (63 %) was the most frequently isolated microorganism; Candida tropicalis (14 %), Candida glabrata (13 %), Candida rugosa (5 %), Candida parapsilosis (3 %), and Candida krusei (2 %) were also detected. The XTT assay showed that C. albicans SC5314 possessed a biofilm-forming ability significantly higher (p < 0.001) than non-albicans Candida strains, after 6 h 37 °C. The total C. albicans CFU from a dual-species biofilm was less than the total CFU of a monospecies C. albicans biofilm. In contrast to the profuse hyphae verified in monospecies C. albicans biofilms, micrographies showed that the C. albicans/non-albicans Candida biofilms consisted of sparse yeast forms and profuse budding yeast cells that generated a network. These results suggested that C. albicans and the tested Candida species could co-exist in biofilms displaying apparent antagonism. The study provide the first description of C. albicans/C. rugosa mixed biofilm.
义齿衬垫的物理特性有利于菌斑积聚和念珠菌属的定植，刺激口腔组织并导致义齿性口炎。为了分离并确定口腔念珠菌属在假牙中的发生率，从66例患者的假牙上采集了口腔拭子。对所有菌株形成生物膜的能力进行了筛选；测试了单菌种和双菌种组合。白色念珠菌（63%）是最常分离出的微生物；还检测到热带念珠菌（14%）、光滑念珠菌（13%）、皱落念珠菌（5%）、近平滑念珠菌（3%）和克柔念珠菌（2%）。XTT分析表明，在37℃培养6小时后，白色念珠菌SC5314形成生物膜的能力明显高于非白色念珠菌菌株（p<0.001）。双菌种生物膜中的白色念珠菌总菌落形成单位少于单菌种白色念珠菌生物膜的总菌落形成单位。与单菌种白色念珠菌生物膜中大量的菌丝不同，显微照片显示白色念珠菌/非白色念珠菌生物膜由稀疏的酵母形式和大量产生网络的出芽酵母细胞组成。这些结果表明，白色念珠菌和受试念珠菌属可以在表现出明显拮抗作用的生物膜中共存。该研究首次描述了白色念珠菌/皱落念珠菌混合生物膜。
