Janeczko Monika, Skrzypek Tomasz
Department of Molecular Biology, Faculty of Medicine, The John Paul II Catholic University of Lublin, Konstantynów 1i, 20-708 Lublin, Poland.
Department of Biomedicine and Environmental Research, Faculty of Medicine, The John Paul II Catholic University of Lublin, Konstantynów 1j, 20-708 Lublin, Poland.
Pathogens. 2025 Jan 8;14(1):40. doi: 10.3390/pathogens14010040.
In this study, we investigated the interactions between and , , , and in mixed infections. Initially, these interactions were studied qualitatively and quantitatively in dual-species biofilms formed in vitro. The MTT assays, determination of the total CFU/mL, and SEM analysis showed that interacted differentially with the other spp. during the dual-species biofilm formation. Depending on the stage of the biofilm development, was found to be a particularly dominant species during its interaction with the biofilms but significantly submissive in the biofilms. These studies were then extended to in vivo host models of experimental candidiasis. larvae were inoculated with monotypic and heterotypic suspensions of . The survival rates and quantification of fungal cells in the hemolymph showed that the highest mortality was exhibited by larvae in the co-infection (100% mortality after 36 h). The CFU/mL values of from the larval hemolymph were lower in the interactive groups compared to the mono-species group. As a newly emerging species, persists in environments in the presence of other species and is involved in both competitive and noncompetitive interactions with other species during biofilm formation and development of experimental candidiasis.
在本研究中，我们调查了[具体物种1]与[具体物种2]、[具体物种3]、[具体物种4]和[具体物种5]在混合感染中的相互作用。最初，这些相互作用在体外形成的双物种生物膜中进行了定性和定量研究。MTT分析、每毫升总菌落形成单位（CFU/mL）的测定以及扫描电子显微镜（SEM）分析表明，在双物种生物膜形成过程中，[具体物种1]与其他[具体物种]的相互作用存在差异。根据生物膜发育阶段，发现[具体物种1]在与[具体物种2]生物膜相互作用时是特别占优势的物种，但在与[具体物种3]生物膜相互作用时明显处于从属地位。这些研究随后扩展到实验性念珠菌病的体内宿主模型。用[具体物种1]的单型和异型悬浮液接种[幼虫种类]幼虫。血淋巴中真菌细胞的存活率和定量分析表明，在[具体物种1]与[具体物种4]共同感染中幼虫的死亡率最高（36小时后死亡率为100%）。与单物种组相比，相互作用组中来自幼虫血淋巴的[具体物种1]的CFU/mL值较低。作为一个新出现的物种，[具体物种1]在存在其他[具体物种]的环境中持续存在，并在生物膜形成和实验性念珠菌病发展过程中与其他[具体物种]发生竞争性和非竞争性相互作用。
