Wang Jialu, Zhu Ying, Jin Feng, Tang Ling, He Zhenwei, He Zhiyi
Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China.
Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China
J Int Med Res. 2016 Jun;44(3):419-32. doi: 10.1177/0300060516630852. Epub 2016 Mar 28.
To measure the differential expression of microRNAs (miRNAs) in peripheral blood samples from patients with intracerebral haemorrhage (ICH) and to measure the levels of hsa-miR-21-5p in peripheral blood and haematoma samples from patients with ICH.
This case-control study enrolled individuals with ICH in the putamen treated by craniotomy and age- and sex-matched healthy control subjects. Serum miRNA expression profiles were determined in the patient and control groups using miRNA polymerase chain reaction (PCR) arrays. The ICH-related miRNA hsa-miR-21-5p was selected and its differential expression was assessed in peripheral blood and haematoma specimens from patients with ICH compared with peripheral blood samples controls using real-time PCR.
Seven patients and five control subjects were included in the miRNA expression profile analysis; and 31 patients and 22 control subjects provided samples for the real-time PCR of hsa-miR-21-5p expression. A total of 59 miRNAs were significantly downregulated in patients with ICH. Relative hsa-miR-21-5p levels of 0.43 and 0.31 for peripheral blood and haematoma samples, respectively, were obtained in the patient group compared with the control subjects.
Hsa-miR-21-5p levels were significantly reduced in both peripheral blood and haematoma samples in patients with ICH.
检测脑出血（ICH）患者外周血样本中微小RNA（miRNA）的差异表达，并检测ICH患者外周血和血肿样本中hsa-miR-21-5p的水平。
本病例对照研究纳入了接受开颅手术治疗的壳核脑出血患者以及年龄和性别匹配的健康对照者。使用miRNA聚合酶链反应（PCR）阵列测定患者组和对照组的血清miRNA表达谱。选择与ICH相关的miRNA hsa-miR-21-5p，并通过实时PCR评估其在ICH患者外周血和血肿标本中的差异表达，与外周血样本对照进行比较。
7例患者和5名对照者纳入miRNA表达谱分析；31例患者和22名对照者提供了用于hsa-miR-21-5p表达实时PCR的样本。ICH患者中共有59种miRNA显著下调。与对照者相比，患者组外周血和血肿样本中hsa-miR-21-5p的相对水平分别为0.43和0.31。
ICH患者外周血和血肿样本中hsa-miR-21-5p水平均显著降低。
