Furuse A, Bernstein J, Welling L W, Welling D J
Department of Anatomic Pathology, William Beaumont Hospital, Royal Oak, MI 48072.
Pediatr Nephrol. 1989 Jul;3(3):265-72. doi: 10.1007/BF00858527.
We studied the morphologic features of renal tubular differentiation in transfilter metanephric culture. Differentiation of portions of the S-shaped loop into proximal convoluted tubule was detected shortly after 72 h of culture by the appearance of microvilli, coated membrane invaginations, and an apical vacuolar-microtubular network. These features developed synchronously, and the microvilli became progressively more numerous and more compact to form a brush border. Computer-assisted morphometric analysis showed only minor differences between proximal tubular cells from 18-day embryos and tubular cells from 7-day cultures of blastema taken from 11-day embryos. Segments of tubule corresponding to distal convoluted tubules were lined with relatively simple cells that contained few differentiating characteristics. Morphometric modeling of the tubular cells indicated a simple shape consistent with an inherent transport function.
我们研究了跨滤器后肾培养中肾小管分化的形态学特征。培养72小时后不久，通过微绒毛、被膜内陷和顶端液泡-微管网络的出现，检测到S形襻的部分分化为近端曲管。这些特征同步发展，微绒毛逐渐变得更多、更紧密，形成刷状缘。计算机辅助形态计量分析显示，来自18天胚胎的近端肾小管细胞与来自11天胚胎的芽基7天培养物中的肾小管细胞之间只有微小差异。对应于远端曲管的肾小管段内衬相对简单的细胞，这些细胞几乎没有分化特征。肾小管细胞的形态计量模型表明其形状简单，与固有的转运功能一致。
