Zuloaga Damian G, Wang Jianming, Weber Sydney, Mark Gregory P, Murphy Stephanie J, Raber Jacob
Department of Behavioral Neuroscience, Oregon Health and Science University, 3181 SW S Jackson Pk Rd, Portland, OR, 97239, USA.
Department of Psychology, University at Albany, SUNY, 1400 Washington Ave, Albany, NY, 12222, USA.
Metab Brain Dis. 2016 Aug;31(4):975-81. doi: 10.1007/s11011-016-9808-z. Epub 2016 Mar 28.
Emerging evidence indicates that methamphetamine (MA) abuse can impact cardiovascular disease. In humans, MA abuse is associated with an increased risk of stroke as well as an earlier age at which the stroke occurs. However, little is known about how chronic daily MA exposure can impact ischemic outcome in either humans or animal models. In the present study, mice were injected with MA (10 mg/kg, i.p.) or saline once daily for 10 consecutive days. Twenty-four hours after the final injection, mice were subjected to transient middle cerebral artery occlusion (tMCAO) for one hour followed by reperfusion. Mice were tested for novel object memory at 96 h post-reperfusion, just prior to removal of brains for quantification of infarct volume using 2,3,5-Triphenyltetrazolium Chloride (TTC) staining. Mice treated with MA prior to tMCAO showed decreased object memory recognition and increased infarct volume compared to saline-treated mice. These findings indicate that chronic MA exposure can worsen both cognitive and morphological outcomes following cerebral ischemia.
新出现的证据表明，甲基苯丙胺（MA）滥用会影响心血管疾病。在人类中，MA滥用与中风风险增加以及中风发生的年龄提前有关。然而，关于慢性每日MA暴露如何影响人类或动物模型中的缺血性结局，人们知之甚少。在本研究中，小鼠连续10天每天腹腔注射MA（10mg/kg）或生理盐水一次。最后一次注射后24小时，对小鼠进行短暂性大脑中动脉闭塞（tMCAO）1小时，随后再灌注。在再灌注后96小时，在移除大脑以使用2,3,5-氯化三苯基四氮唑（TTC）染色定量梗死体积之前，对小鼠进行新物体记忆测试。与生理盐水处理的小鼠相比，在tMCAO之前用MA处理的小鼠表现出物体记忆识别能力下降和梗死体积增加。这些发现表明，慢性MA暴露会使脑缺血后的认知和形态学结局恶化。
