Hong Yun Ji, Chung Yousun, Hwang Sang Mee, Park Jeong Su, Kwon Jeong-Ran, Choi Young Sill, Kim Jun Nyun, Lee Dong Han, Kwon So-Yong, Cho Nam-Sun, Song Eun Young, Park Kyoung Un, Song Junghan, Han Kyou Sup
Department of Laboratory Medicine, Seoul National University Bundang Hospital, 82, Gumi-ro 173 beon-gil, Bundang, Seongnam, 13620, Gyeonggi, Korea.
Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
Ann Hematol. 2016 May;95(6):985-91. doi: 10.1007/s00277-016-2645-7. Epub 2016 Mar 28.
It is often difficult for standard blood banks in Korea to supply adequate amounts of blood for patients with rare phenotype. Moreover, the definition of a blood in need is ambiguous, and much remains to be learned. In this study, we determined the prevalence of various red blood cell (RBC) antigens from a donor viewpoint and estimated the demand for specific antigen-negative blood from a patient viewpoint. Our data will aid the establishment of a Rare Blood Program in Korea (KRBP). RBC genotyping of 419 blood donors was performed using a Lifecodes RBC/RBC-R typing kit (Immucor, Norcross, GA). A national recipient registry website has been established. Each hospital-based blood bank voluntarily enters data on antibodies detected and identified and the outcomes of specific antigen testing. We calculated the availabilities of specific antigen-negative blood components based on these registry data and predicted the prevalence of RBC antigens via RBC genotyping. The prevalences of various RBC antigens in the D-negative population were determined for the first time, and the Cartwright, Scianna, Dombrock, Colton, Landsteiner-Wiener, Cromer, and Knops blood group systems were identified. The availabilities of specific antigen-negative units differed when calculations were based on serotyping or genotyping, especially in the D-negative group. Data on the prevalences of various blood antigens are essential for estimating the availabilities of blood components that are appropriate for use by patients expressing relevant antibodies. Then, blood banks would be able to efficiently supply safe blood products.
韩国的标准血库往往难以向具有罕见血型的患者提供足够的血液。此外，急需用血的定义尚不明确，仍有许多有待了解的地方。在本研究中，我们从献血者的角度确定了各种红细胞（RBC）抗原的流行率，并从患者的角度估计了对特定抗原阴性血液的需求。我们的数据将有助于在韩国建立一个稀有血型计划（KRBP）。使用Lifecodes RBC/RBC-R分型试剂盒（Immucor，诺克罗斯，佐治亚州）对419名献血者进行红细胞基因分型。已经建立了一个全国受血者登记网站。每个医院血库自愿输入检测和鉴定出的抗体数据以及特定抗原检测的结果。我们根据这些登记数据计算了特定抗原阴性血液成分的可获得性，并通过红细胞基因分型预测了红细胞抗原的流行率。首次确定了D阴性人群中各种红细胞抗原的流行率，并鉴定出了卡特赖特、斯恰纳、多姆布罗克、科尔顿、兰德施泰纳-维纳、克罗默和诺普斯血型系统。当基于血清分型或基因分型进行计算时，特定抗原阴性单位的可获得性有所不同，尤其是在D阴性组中。各种血液抗原流行率的数据对于估计适合表达相关抗体的患者使用的血液成分的可获得性至关重要。这样，血库就能有效地提供安全的血液制品。
